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Preparation method of high-concentration KT gel

A KT gel and preparation method technology, applied in the field of plant biology, can solve the problems of loss of branches, high cost, unsatisfactory production effect, etc., and achieve the effects of reducing water evaporation and easy operation.

Inactive Publication Date: 2015-10-28
钱家静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Experimental program
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Embodiment

[0011] A preparation method of high-concentration KT gel, characterized in that, the method includes the preparation of hormone mother solution, the preparation of working solution, and the preparation of hormone gel, and the specific operation steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of KT and dissolve it with a little 1M KOH, then dilute to 100mL with distilled water, which is 1mg / mL KT mother solution;

[0013] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:2 into a high-concentration hormone working solution with a concentration of 500mg / L;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone working solution with a concentration of 500 mg / L in a water bath at 50 °C for later use.

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Abstract

The invention discloses a preparation method of a high-concentration KT gel, belonging to the technical field of plant biology. Firstly KT mother solution of 1mg / mL is prepared; the mother solution is diluted into high-concentration KT working solution of 500 mg / L; by using the working solution, high-concentration KT gel confining liquid of sodium carboxymethylcellulose with the mass concentration of 1 percent and sodium alginate with the mass concentration of 8 percent is prepared.

Description

technical field [0001] A preparation method of high-concentration KT gel belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to dea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/90A01P21/00
Inventor 钱家静
Owner 钱家静