Molecular marker related to porcine reproduction traits based on PGR genes and detection method and application of molecular marker

A technology of molecular markers and detection methods, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no PGR gene and other problems, and achieve the effect of easy detection

Inactive Publication Date: 2015-12-09
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Molecular marker related to porcine reproduction traits based on PGR genes and detection method and application of molecular marker
  • Molecular marker related to porcine reproduction traits based on PGR genes and detection method and application of molecular marker
  • Molecular marker related to porcine reproduction traits based on PGR genes and detection method and application of molecular marker

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Embodiment 1

[0028] In 2013, the ear samples of 106 Xiaomeishan pigs, 42 Fengjing pigs and 70 Large White pigs were collected; at the same time, the ear samples of the above 106 Xiaomeishan sows from 2004 to 2012 were sorted out Production files, a total of 698 litter piglet records, mainly collect indicators such as farrowing year, parity, TNB, NBA and so on. Ear samples (about 0.15 g) were collected with ear size forceps and frozen at -20°C. The genomic DNA of the pig ear sample was extracted by conventional phenol-chloroform extraction method, the OD value of the genomic DNA was measured, the concentration was calculated, and the ddH 2 Dilute O to a final concentration of 100ng / μL, store the original solution at -20°C, and store the diluted solution at 4°C.

[0029] Step 1: Obtain the exon sequence of the porcine PGR gene.

[0030] According to the complete sequence of the human PGR gene published in GenBank (accession number: AY525610.1) and the porcine PGR mRNA sequence (accession n...

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Abstract

The invention discloses a molecular marker related to porcine reproduction traits based on PGR genes and a detection method and application of the molecular marker. The molecular marker is a porcine PGR gene segment, the porcine PGR gene segment includes a polymorphic site, and the polymorphic site is, for example, the 1319th in a sequence shown by SEQ ID NO.1, and has variations of C/T basic groups. The detection method comprises the steps that a primer is designed according to a nucleotide sequence of the molecular marker; PCR amplification is carried out with a genome DNA of a pig to be detected serving as a template, and the type of the polymorphic site in a PCR amplification product is judged. The application of the molecular marker in porcine reproduction trait selection is further disclosed. The molecular marker related to the porcine reproduction traits based on the PGR genes and the detection method and application of the molecular marker are provided according to a PGR gene mutation site, and a foundation is provided for porcine molecular breeding and study of porcine reproduction physiological mechanisms.

Description

technical field [0001] The invention relates to the field of animal molecular breeding, in particular to a PGR gene-based molecular marker related to pig reproductive traits and a detection method and application thereof. Background technique [0002] Molecular marker-assisted selection is an important application of genetic engineering in modern livestock breeding. It uses various markers related to breeding traits instead of phenotype-based selection, and analyzes the genetic composition of individuals at the molecular level, so as to achieve gene selection. Type of direct selection can overcome the disadvantages of direct selection, improve the accuracy of selection and shorten the generation interval. [0003] The reproductive traits of pigs, such as total litter size and live piglet size, are important economic traits, which are directly related to the economic benefits of pig farming. [0004] Progesterone is a very important steroid hormone, which plays a key role in...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴井生陈超郭苹陈永霞
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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