Method for evaluating polygonum multiflorum thunb idiosyncratic hepatotoxicity

A Polygonum multiflorum, idiosyncratic technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as incomplete clarity, and achieve the effects of short time, low economic cost, and easy operation.

Active Publication Date: 2016-04-06
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mechanism of idiosyncratic hepatotoxicity is still not completely clear. Many studies have shown that the combination of endotoxins such as bacterial lipopolysaccharide (lipopolysaccharide, LPS) immunization pre-intervention in animal models is similar to clinical drug idiosyncratic hepatotoxicity.
However, there is no report on whether Polygonum multiflorum can be used for idiosyncratic hepatotoxicity evaluation using LPS

Method used

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  • Method for evaluating polygonum multiflorum thunb idiosyncratic hepatotoxicity
  • Method for evaluating polygonum multiflorum thunb idiosyncratic hepatotoxicity
  • Method for evaluating polygonum multiflorum thunb idiosyncratic hepatotoxicity

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Experimental program
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Embodiment 1

[0053] Polygonum multiflorum (purchased from Beijing Lvye Pharmaceutical Co., Ltd., batch number 13101701, place of origin: Hubei).

[0054] Lipopolysaccharide (lipopolysaccharide, LPS) was purchased from Sigma Company (lot number L2880, 100 mg / cartridge). Sodium pentobarbital (Sigma company). ALT and AST kits were purchased from Nanjing Jiancheng Biotechnology Co., Ltd., batch number 20140420. All other reagents used were of analytical grade.

[0055] Wherein, in the microplate reader method described below, a kit is used for testing.

[0056] The SPSS software used below is SPSS13.0 software.

[0057] Hereinafter, Polygonum multiflorum is represented by PM.

[0058] And, the dried extract of Polygonum multiflorum used hereinafter was prepared by:

[0059] Weigh 1000g of Polygonum multiflorum decoction pieces with a diameter of about 1cm, add 8000ml of ethanol with a volume percentage of 50% for cold extraction, wherein the volume of ethanol in milliliters is 8 times the...

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Abstract

The present application provides a method for evaluating polygonum multiflorum thunb idiosyncratic hepatotoxicity. The method comprises: a, polygonum multiflorum thunb solution preparation; b, endotoxin solution preparation; c, animal experiments; d, index detection; and e, statistical analysis. According to the present invention, with the method based on the LPS, the polygonum multiflorum thunb idiosyncratic hepatotoxicity can be rapidly evaluated, and the reference can be provided for the polygonum multiflorum thunb hepatotoxicity evaluation model, the scientific mechanism research, and the clinical application.

Description

technical field [0001] This application relates to a method for evaluating the idiosyncratic hepatotoxicity of Polygonum multiflorum Background technique [0002] Polygonum multiflorum is the root of Polygonum multiflorum Thunb., a plant of the Polygonaceae family. It is a famous traditional Chinese medicine for tonic. It is widely used in liver and kidney, black hair, Chinese medicine preparations for lowering blood lipids, health food, public daily meals, diet and health care, and hair care. Supplies etc. However, in recent years, there has been a large increase in the reports of adverse reactions of Polygonum multiflorum at home and abroad, including taking raw Polygonum multiflorum, prepared Polygonum multiflorum, compound containing Polygonum multiflorum, Chinese patent medicine or health food, etc. Domestic and foreign literature reports about 150 cases of liver damage in Polygonum multiflorum. In 2006, the British and Australian drug regulatory authorities successiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/52
Inventor 王伽伯肖小河李春雨马致洁赵艳玲柏兆方
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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