A Molecular Marker Linked to Fiber Length of Cotton Sea Island Cotton

A technology of cotton fiber and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, plant gene improvement, etc., can solve the problems of slow progress, poor accuracy, and high cost of fiber quality breeding, and achieve the goal of solving slow progress, The effect of improving accuracy and efficiency and speeding up the breeding process

Active Publication Date: 2019-10-22
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: in order to overcome many shortcomings such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost in traditional breeding, and to solve the problem of slow progress in fiber quality breeding, the present invention provides a kind of high-quality material from fiber Molecular markers linked to cotton fiber length QTL / main effect gene of Sea Island Cotton Sea 1, that is, by screening the SSR molecular markers linked to fiber length QTL / main effect gene in Sea Island Cotton Sea 1 from the high-quality fiber material, the DNA level Early marker-assisted selection to improve breeding efficiency

Method used

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  • A Molecular Marker Linked to Fiber Length of Cotton Sea Island Cotton
  • A Molecular Marker Linked to Fiber Length of Cotton Sea Island Cotton
  • A Molecular Marker Linked to Fiber Length of Cotton Sea Island Cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Screening Molecular Markers

[0035] (1) Construction of chromosome segment substitution lines and acquisition of phenotypic data

[0036] The inventors used Sea Island Mianhai 1 as the donor parent and Zhongmiansuo 45 as the recipient parent to construct a set of chromosome segment substitutions with Zhongmiansuo 45 as the genetic background through hybridization, high-generation backcrossing, and continuous self-crossing. Department, awarded 116 BCs in 2007 4 f 1 Family (Yang Zemao et al., 2009). The donor parent Hai 1 (Hai1) is a sea-island cotton cultivar with high fiber quality and a dominant non-glandular gene, and Zhongmian Institute 45 (National Cotton 2003002) is a high-yielding, resistant Mid-maturing variety with lodging and resistance to Verticillium wilt. .

[0037] 116 BC planted in 2009 4 f 3 In the family line, Zhongmiansuo 45 was used as a control, with a row length of 5m and a plant spacing of 25cm. There are about 20 plants in each f...

Embodiment 2

[0061] Example 2 Molecular marker selection method for improving fiber length traits of upland cotton

[0062] Using the molecular marker CGR5242 obtained in Example 1 100 and NAU2977 150 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0063](1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Lumianyan 28, Zhongmian Institute 60) as the recipient parent, perform hybridization, backcrossing, and selfing to obtain segregated populations , or the chromosomal segment substitution line and its derivative line obtained by high-generation backcrossing with Haidao Mianhai 1 as the donor parent and upland cotton varieties (such as Lumianyan 28 and Zhongmiansuo 60) as the recipient parent, or other Chromosomal segment substitution lines and upland cotton varieties are crossed and backcrossed, and the DNA of individual plants ...

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Abstract

The invention discloses a molecular marker linked with cotton fiber length QTL / major genetic loci qFL-C13-1 and qFL-C22-1. The qFL-C13-1 and the qFL-C22-1 are positioned on a chromosome C13 and a chromosome C22 respectively. A molecular marker tightly linked with the qFL-C13-1 is CGR5242100, and a molecular marker tightly linked with the qFL-C22-1 is NAU2977150. The SSR markers linked with the cotton fiber length QTL / the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber length breeding efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker linked with cotton sea-island cotton fiber length and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Fiber quality breeding has become one of the main goals of cotton breeding in my country. Fiber length is an important indicator of fiber quality traits. Breeding varieties with longer fibers is one of the requirements for spinning high-grade yarns and producing high-grade cotton fabrics, and is also an important requirement for mechanized cotton harvesting. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
Inventor 石玉真袁友禄檀运娜苏威马留军孔令磊刘爱英李俊文巩万奎龚举武商海红陈婷婷葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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