A processing box with liquid nitrogen cryopreservation and oct embedding functions for rat spinal cord

Abstract

The invention discloses a treatment box with rat spinal cord liquid nitrogen freezing storage and OCT (optimal cutting temperature) embedding functions. The treatment box comprises a treatment box body. The treatment box is characterized in that the treatment box body is a hollow cuboid; a part, in contact with a rat spinal cord sample, of an internal space of the hollow cuboid is aseptic; the length of the hollow cuboid is 1.2 times that of the adult rat spinal cord and the cross section area of the hollow cuboid is 2.5 times that of the rat spinal cord; an aseptic sample tank is arranged in the treatment box body; a floating fixed wing is connected to the lower part of the aseptic sample tank; the center of the floating fixed wing protrudes downwards and the periphery of the floating fixed wing warps upwards; a sample mark reserved region is arranged on the floating fixed wing; a transverse reserved gap and a longitudinal reserved gap are formed in the treatment box body; and a transverse easy-pull line and a longitudinal easy-pull line are arranged in the transverse reserved gap and the longitudinal reserved gap respectively. The treatment box is reasonable in structure; and the rat spinal cord can be subjected to one-stop treatment of sample storage, liquid nitrogen quick freezing and OCT embedding, so that the labor efficiency of experimental workers is improved and the phenomena of sample damage and experimental result errors caused by an improper storage tool are reduced.

Description

technical field [0001] The invention relates to a biological experiment appliance, in particular to a processing box which has the functions of liquid nitrogen cryopreservation and OCT embedding of rat spinal cord. Background technique [0002] Due to its length and flexibility, the rat spinal cord is not suitable for cryopreservation in the current commercially available cryopreservation tubes and EP tubes. Commercially available liquid nitrogen cell cryopreservation tubes and EP tubes are more suitable for microscopic samples or samples prepared into liquids such as cells, E. Samples with unchanged physicochemical properties such as bone, skin and hair. However, the softness of the rat spinal cord is easily affected by the external force of common surgical equipment such as scissors and tweezers, resulting in damage or deformation of the sample, which will have a great impact on downstream experiments, especially for slice analysis of pathology and external damage experim...

AI Technical Summary

Problems solved by technology

[0003] Before frozen sectioning of rat spinal cord samples, OCT embedding agent needs to be used for embedding. Due to the length of the sample body, the spinal cord needs to be embedded upright when performing coronal sectioning, which is very inconvenient. When buried, it is inevitable that there will be secondary damage to the operating tools or repeated freezing and thawing, which will have a great impact on frozen sections and subsequent analysis of results

Images