The invention discloses an anti-rollover biological floating bed for river regulation and an implementation method thereof. The anti-rollover biological floating bed for river regulation comprises a floating mechanism and a protection mechanism, arc-shaped baffles are installed on the two sides of a floating bed body, and air inducing grooves are formed in the upper ends of the sides, away from the floating bed body, of the arc-shaped baffles; and the arc-shaped baffles can shield the floating bed body and planting baskets, the air inducing grooves enable shielded wind power or wave water to flow out through the air inducing grooves, a balancing weight and a gravel layer are arranged at the bottom end of the inner cavity of a semicircular base, and a foam filling layer and a sealing hollow layer are arranged at the upper end of the inner cavity of the semicircular base. Therefore, the lower end of the semicircular base is heavy, and the upper end of the semicircular base is light. According to the principle of a tumbler, the semicircular base and the floating bed body cannot be blown over, so that the floating bed body keeps stable on a river channel, an impurity collecting box collects floating garbage on the water surface through a collecting empty groove, and a limiting baffle can block the floating garbage on the river channel, so that the floating garbage moves downwards to enter the inner side of the collecting empty groove.