The invention relates to a method for removing salt from an
antimicrobial lipopeptide solution in a low temperature
refrigeration environment. The method comprises 1, carrying out
bacillus amyloliquefaciens fermentation for
antimicrobial lipopeptide preparation, filtering the
fermentation broth, and removing
bacteria so that a clear liquor is obtained, 2, feeding diluted
hydrochloric acid into the clear liquor, carrying out standing at a
room temperature, and removing a supernatant so that
slurry-form fluid is obtained, 3, adding an extractant into the
slurry-form fluid, carrying out uniform stirring, carrying out extraction and
centrifugation, and carrying out
microfiltration by an organic membrane to obtain filtrate, and 4, carrying out
crystallization on the filtrate at a temperature of -25 to -10 DEG C, and carrying out
microfiltration to remove crystals so that an
antimicrobial lipopeptide solution is prepared. In a low temperature
refrigeration storage environment, the inorganic salt is fully precipitated from the extraction
system, and the organic solution subjected to
precipitation and
microfiltration does not crystallize in low temperature
refrigeration storage so that the problem that
impurity salts produced by the existing antimicrobial lipopeptide preparation process are removed difficultly is solved.