A method for desalting from antimicrobial lipopeptide solution using low-temperature freezing environment

Abstract

The invention relates to a method for removing salt from an antimicrobial lipopeptide solution in a low temperature refrigeration environment. The method comprises 1, carrying out bacillus amyloliquefaciens fermentation for antimicrobial lipopeptide preparation, filtering the fermentation broth, and removing bacteria so that a clear liquor is obtained, 2, feeding diluted hydrochloric acid into the clear liquor, carrying out standing at a room temperature, and removing a supernatant so that slurry-form fluid is obtained, 3, adding an extractant into the slurry-form fluid, carrying out uniform stirring, carrying out extraction and centrifugation, and carrying out microfiltration by an organic membrane to obtain filtrate, and 4, carrying out crystallization on the filtrate at a temperature of -25 to -10 DEG C, and carrying out microfiltration to remove crystals so that an antimicrobial lipopeptide solution is prepared. In a low temperature refrigeration storage environment, the inorganic salt is fully precipitated from the extraction system, and the organic solution subjected to precipitation and microfiltration does not crystallize in low temperature refrigeration storage so that the problem that impurity salts produced by the existing antimicrobial lipopeptide preparation process are removed difficultly is solved.

Description

technical field [0001] The invention relates to a method for removing salt from an antibacterial lipopeptide solution by using a low-temperature freezing environment, and belongs to the technical field of antibacterial peptide purification. Background technique [0002] Antimicrobial lipopeptide is a kind of antimicrobial peptide, which is generally a metabolite produced by Gram-positive bacilli. The molecule of antimicrobial lipopeptide is composed of two parts: hydrophilic peptide bond and lipophilic aliphatic hydrocarbon chain. Due to its special chemical composition and amphiphilic molecular structure, antimicrobial lipopeptide has the characteristics of surfactant in addition to antibacterial activity. Antimicrobial lipopeptides have important application prospects in the fields of biomedicine, agriculture, oil exploration, food and cosmetics, and environmental governance, and have attracted widespread attention. [0003] Antibacterial lipopeptides that have been disco...

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Problems solved by technology

[0013] However, the above method is only suitable for laboratory verification, and there are problems that it cannot be applied on a large scale and the toxicity of organic solvents is high. Moreover, due to the inevitable addition of a small amount of water during the extraction process, the presence of salt makes the antibacterial lipopeptides in the storage process. It is easier to hydrolyze, and it is prone to crystallization and precipitation during the transition to other organic phases; there is a tailing phenomenon in qualitative detection, so the purity of the final product is not high

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