A storage box with the functions of liquid nitrogen cryopreservation and oct embedding of rat brain

Abstract

The invention relates to a preservation box with rat brain liquid nitrogen cryopreservation and OCT embedding functions. The preservation box comprises a preservation box body which is a cylinder. A part of the internal main space of the preservation body that contacts a rat brain sample is sterile. The length of the preservation box body is 1.2 times that the length of an adult rat brain. The cross-section area of the preservation box body is 1.5 times that the cross-section area of a rat brain. A sterile sample slot is arranged in the preservation box body. A floating base is connected under the preservation box body. The center of the floating base is protruding downwards, and the periphery of the floating base is upturned. A sample marking reserved zone is arranged on the floating base. A transverse reserved seam and a longitudinal reserved seam are arranged on the preservation box body. A transverse easy-pulling string and a longitudinal easy-pulling string are respectively arranged in the transverse reserved seam and the longitudinal reserved seam. With the preservation box, one-stop processing of rat brain sampling and preservation, liquid nitrogen quick freezing and OCT embedding can be realized, such that experimenter labor efficiency can be improved, and phenomena of sample damage and experimental result error caused by improper preservation tools are reduced.

Description

technical field [0001] The invention relates to a preservation box, in particular to a preservation box with the functions of liquid nitrogen cryopreservation and OCT embedding of rat brains. Background technique [0002] After the rat brain is collected, because its material is soft and the cell components are not easy to retain, its size is larger than that of ordinary mouse brain, so it is not suitable to use the current cryopreservation tubes and EP tubes on the market for cryopreservation. Commercially available liquid nitrogen cell cryopreservation tubes and EP tubes are more suitable for microscopic samples or samples prepared into liquids such as cells, bacteria or protein lysates, etc., or are relatively strong, not easy to deform or deformed. The structure and physical and chemical properties Unaltered samples such as bone, skin, and hair. But for the softness of the rat brain, it is easily affected by the external force of common equipment such as scissors and tw...

AI Technical Summary

Problems solved by technology

[0003] Before the frozen section of the rat brain sample, OCT embedding agent needs to be used for embedding, and the brain is generally coronal sectioned, which requires the rat brain to be upright when embedding and taken out in the EP tube or cryopreservation tube When the sample is embedded, it is inevitable that there will be secondary damage to the operating tool or repeated freezing and thawing, which will have a great impact on the frozen section and the analysis of the later results.

Images