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Method for testing contents of multiple components of medicinal material inula cappa

A method of determination, the technology of sheep ear chrysanthemum, which is applied in the direction of material separation, measuring devices, and analysis of materials, can solve the problems of not fully reflecting the internal quality, etc., and achieve the effects of high sensitivity, improved efficiency, and strong specificity

Active Publication Date: 2016-06-01
GUIZHOU MEDICAL UNIV
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Problems solved by technology

"Quality Standards for Traditional Chinese and Ethnic Medicines of Guizhou Province" has not included the content determination items of Goat Fructus, and after reviewing the literature, only the single component and total flavonoids of Goat Fructus are determined, which cannot fully reflect its internal quality

Method used

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  • Method for testing contents of multiple components of medicinal material inula cappa
  • Method for testing contents of multiple components of medicinal material inula cappa
  • Method for testing contents of multiple components of medicinal material inula cappa

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Embodiment Construction

[0016] In order to describe the present invention more clearly, the specific implementation manners of the present invention will be described in more detail below through specific implementation examples. However, it should be understood that the specific implementation examples described below are only used to illustrate the present invention, and are not used to limit the present invention, and the materials, reagents, instruments and operating conditions used are only representative and are not intended to be used for the present invention. limited to the cases listed. Those skilled in the art can make changes and improvements to the present invention without departing from the protection scope defined by the claims of the present invention by reading the following descriptions, and these changes and improvements are also within the protection scope of the present invention.

[0017] 1 Instruments and reagents

[0018] 1.1 Instrument

[0019] ACQUITY system ultra-high pe...

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Abstract

The invention discloses a method for testing contents of multiple components of a medicinal material inula cappa. The method comprises the following step: testing contents of 6 components, namely, scopoli, 1,3-O-dicaffeoyl-quinic acid, galuteolin, 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid and 4,5-O-dicaffeoyl-quinic acid in the medicinal material inula cappa by using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), wherein puerarin is taken as internal standard in ultra-high performance liquid chromatography-tandem mass spectrometry analysis. The method for testing the contents of multiple components of the medicinal material inula cappa is established for a first time, the contents of the 6 components in inula cappa can be accurately tested at the same time within 6 minutes through UPLC-MS / MS, and a rapid, sensitive, stable and reliable novel method is provided for quality control on inula cappa.

Description

technical field [0001] The invention relates to the technical field of identification and quality detection of medicinal components, in particular to a method for determining the content of various components in the medicinal material of chamomile. Background technique [0002] Sheep's ear chrysanthemum is the dried whole herb of Inulacappa (Buch.-Ham.exD.Don) DC., a plant of Compositae. It has the effects of dispelling wind and heat, detoxifying and reducing swelling. Bexniouxdant" (approximate Chinese transliteration is "white ox gallbladder"), which is recorded in "Miao Medicine", "Chinese Materia Medica Miao Medicine Volume", 2003 edition "Guizhou Provincial Traditional Chinese Medicine and Ethnic Medicine Quality Standards", Guizhou Province Various Miao medicine preparations such as "Qishenhoubitong Granules", "Bikang Tablets" and "Yanggan Dropping Pills" developed with sheep's ear chrysanthemum as raw materials have been marketed one after another, with remarkable cur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 兰燕宇黄勇李勇军王永林郑林王爱民廖尚高孙佳李月婷陈思颖陆苑巩仔鹏
Owner GUIZHOU MEDICAL UNIV
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