Circulating tumor cell separation device adopting combined field flow separation

A field flow separation and tumor cell technology, which is applied in the field of combined field flow separation circulating tumor cell separation devices, can solve the problems of small force and poor efficiency, and achieves the advantages of improving efficiency, speeding up processing speed and large processing throughput. Effect

Active Publication Date: 2016-06-08
上海揽微赛尔生物科技有限公司
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Problems solved by technology

Dielectrophoresis can realize particle manipulation in the horizontal and vertical planes, but it has the disadvantages of small force and poor efficiency

Method used

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  • Circulating tumor cell separation device adopting combined field flow separation
  • Circulating tumor cell separation device adopting combined field flow separation

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

[0022] Such as figure 1 As shown, the separation device of the present invention adopts surface acoustic wave and dielectrophoresis-driven field flow separation to realize the removal of red blood cells. When the surface acoustic wave is perpendicular to the cell flow direction, the blood cells under the action of the acoustic wave field gather in the lateral direction according to the different volumes. Selecting an appropriate driving signal can make red blood cells gather on one side, while larger white blood cells gather on one side, realizing the separation of red blood cells. Dielectrophoretic electrodes generate a non-uniform electric field that can drive cell aggregation in the vertical plane, placing various cells in zones with different heights. Since the subsequent recogni...

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Abstract

The invention provides a circulating tumor cell separation device adopting combined field flow separation. The circulating tumor cell separation device comprises an upper cover plate, a channel plate and a substrate plate from top to bottom; a two-step separation method comprising the field flow separation and specific molecular affinity identification is adopted, wherein the field flow separation adopts an acoustic surface wave driving mode and a dielectrophoresis driving mode, horizontal cell grouping migration and cell layering in a vertical plane are realized respectively, and erythrocytes are preliminarily separated from other cell populations; a residual leucocyte mixed liquid passes through a cell recognition area, circulating tumor cells are fixed in the recognition area by recognition ligands, and a tumor cell sample is acquired through releasing operation finally. The device can rapidly realize circulating tumor cell separation without marks, has great significance in the aspects of patient's disease diagnosis, therapy monitoring, pathological research and the like, and can be used for a separation process of other cells, macromolecular protein and micro-particles.

Description

【Technical field】 [0001] The invention relates to a microfluidic device capable of rapidly separating peripheral blood cells, in particular to a combined field flow separation circulating tumor cell separation device. 【Background technique】 [0002] Circulating tumor cells are tumor cells that fall off from the original tumor tissue and infiltrate into the blood circulation system, and circulate in the body with the blood. Although people still lack a clear understanding of the mechanism and process of cancer metastasis, the role of circulating tumor cells is directly related to cancer metastasis, which has been consistently confirmed. Therefore, the number and morphology of circulating tumor cells can be used as the basis for predicting the development of the patient's disease, such as the diagnosis of the disease, postoperative monitoring, pathological research, etc. However, it is very difficult to obtain tumor cells from peripheral blood samples. There are two main rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
CPCC12M23/16C12M35/02C12M35/04C12M45/07
Inventor 王小章王朝晖张群明
Owner 上海揽微赛尔生物科技有限公司
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