Cell preparation for repairing ovarian functions

A cell preparation and ovarian function technology, applied in the field of cell engineering, can solve problems such as unstable and incomplete repair, and achieve the effect of repairing and thoroughly repairing the ovary

Inactive Publication Date: 2016-06-15
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a mixed stem cell preparation capable of completely repairing ovarian function in view of the shortcomings of unstable and incomplete repair of the ovary in the prior art using a single cell preparation

Method used

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  • Cell preparation for repairing ovarian functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The cell preparation for repairing ovarian function provided by the present invention includes the following components; and in the cell preparation, the concentration of each component is as follows:

[0063] Amniotic mesenchymal stem cells 2×10 5 a / ml;

[0064] Ovary cells 6×10 5 a / ml;

[0065] 15ng / ml heparin;

[0066] 15ng / ml interleukin-6;

[0067] 15ng / ml interleukin-3;

[0068] 50ng / ml stem cell growth factor;

[0069] 50ng / ml FMS-like tyrosine kinase 3 ligand;

[0070] 5% hemoglobin saline injection;

[0071] 5ng / ml fibroblast growth factor;

[0072] 10ng / ml chitosan;

[0073] 200000 units / ml penicillin, volume 20μl;

[0074] 200000 units / ml streptomycin in a volume of 20 μl.

[0075] Among them, the source process of amniotic mesenchymal stem cells includes the following steps:

[0076] S1a, obtaining amniotic membrane;

[0077] S11a. With the approval of the Hospital Ethics Committee and signed informed consent, normal full-term cesarean section w...

Embodiment 2

[0096] The cell preparation provided in this example for repairing ovarian function includes the following components, and the concentration of each component in the cell preparation is as follows:

[0097] Amniotic mesenchymal stem cells 1×10 5 a / ml;

[0098] Ovary cells 4×10 5 a / ml;

[0099] 15ng / ml heparin;

[0100] 10ng / ml interleukin-6;

[0101] 10ng / ml interleukin-3;

[0102] 30ng / ml stem cell growth factor;

[0103] 10ng / ml FMS-like tyrosine kinase 3 ligand;

[0104] 1% hemoglobin saline injection;

[0105] 1ng / ml fibroblast growth factor;

[0106] 5ng / ml chitosan;

[0107] 100000 units / ml penicillin, volume 10μl;

[0108] 100000 units / ml streptomycin in a volume of 10 μl.

[0109] Wherein, the source process of amniotic mesenchymal stem cells and ovarian cells is basically the same as that of Example 1.

Embodiment 3

[0111] The cell preparation provided in this example for repairing ovarian function includes the following components, and the concentration of each component in the cell preparation is as follows:

[0112] Amniotic mesenchymal stem cells 5×10 5 a / ml;

[0113] Ovary cells 8×10 5 a / ml;

[0114] 20ng / ml heparin;

[0115] 20ng / ml interleukin-6;

[0116] 20ng / ml interleukin-3;

[0117] 70ng / ml stem cell growth factor;

[0118] 150ng / ml FMS-like tyrosine kinase 3 ligand;

[0119] 5% hemoglobin saline injection;

[0120] 10ng / ml fibroblast growth factor;

[0121] 20ng / ml chitosan;

[0122] 300000 units / ml penicillin, volume 30μl;

[0123] 300000 units / ml streptomycin, volume 30μl;

[0124] Wherein, the source process of amniotic mesenchymal stem cells and ovarian cells is basically the same as that of Example 1.

[0125] In order to further verify the significant effect of the cell preparation provided by the present invention for repairing ovarian function, the following ...

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Abstract

The invention relates to a cell preparation for restoring ovarian function, including amniotic mesenchymal stem cells and ovarian cells. By using amniotic mesenchymal stem cells combined with ovarian cells to make cell preparations, as well as auxiliary components for preventing the multidirectional differentiation of amniotic mesenchymal stem cells and promoting the activity and normal growth and proliferation of amniotic mesenchymal stem cells and ovarian cells, at the same time, Combined with ovarian cells, amniotic mesenchymal stem cells can maintain their stem cell characteristics for a long time, and then act on the ovary through the cytokines secreted by them combined with ovarian cells to achieve the purpose of immediate and complete restoration of ovarian function; avoid cells made of single cells In the preparation, the cell properties are unstable, resulting in unstable ovarian function repair effect, poor repair effect, and failure to completely repair ovary and other defects.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a cell preparation for restoring ovarian function. Background technique [0002] Ovarian hypofunction refers to the weakening of the ability of the ovaries to produce eggs and the decline in the quality of follicles, resulting in decreased female fertility and lack of sex hormones. Ovarian hypofunction will further develop and lead to ovarian failure. Premature ovarian failure refers to a disease characterized by amenorrhea, infertility, estrogen deficiency and elevated gonadotropin levels caused by women before the age of 40. So far, except for the physiological ovarian hypofunction caused by age, the etiology of most pathological ovarian hypofunction is still unclear. Clinically, the treatment effect is not good, and the success rate of assisted reproductive therapy is also low. [0003] At present, studies have shown that a single preparation made of stem cells can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/54A61K45/06A61K38/42A61P15/08A61K38/18A61K38/20A61K31/727A61K35/28A61K31/722A61K31/43A61K31/7036
CPCA61K35/28A61K31/43A61K31/7036A61K31/722A61K31/727A61K35/54A61K38/18A61K38/1825A61K38/202A61K38/204A61K38/42A61K45/06A61K2300/00
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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