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Stab strain slant low-temperature preserving method

A strain preservation and low-temperature technology, which is applied in the field of low-temperature strain preservation on an inclined plane, can solve the problems of strain death, failure to develop, shape, colony, and virulence that are prone to variation, and achieve the effect of easy use and simple operation

Inactive Publication Date: 2016-06-22
雷印平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Bacteria are an indispensable experimental material in the teaching of microbiology experiments, and microbiology experiments cannot be carried out without it
Therefore, the laboratory has preserved various strains commonly used in teaching for a long time, but these strains are affected by the external environment and their own metabolism. Easy to mutate and become atypical strains or even die

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0010] Inoculate the pure Staphylococcus bacteria on an ordinary agar slant medium, culture it at 37 degrees for 18 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

Embodiment 2

[0012] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0013] Inoculate the purified fungus to be stored on the slant medium of Sabao weak, cultivate it at 37 degrees for 24 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

Embodiment 3

[0015] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0016] Inoculate the pure hemophilic bacteria to be stored on the blood agar slant medium, culture it at 37 degrees for 21 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

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PUM

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Abstract

The invention discloses a stab strain slant low-temperature preserving method. The method comprises the following steps: inoculating separated and purified bacteria to be preserved to a suitable solid slant medium, culturing at 37DEG C for 18-24h, sealing with a sealing membrane, and preserving the sealed bacteria in a 4DEG C refrigerator. The method has the advantages of simple operation, use convenience, no need of a special device, and suitableness for laboratory strain preservation.

Description

technical field [0001] The invention relates to the field of strain preservation, in particular to a low-temperature strain preservation method on an inclined plane. Background technique [0002] Bacteria is an indispensable experimental material in the teaching of microbiology experiments, and microbiology experiments cannot be carried out without it. Therefore, the laboratory has preserved various strains commonly used in teaching for a long time, but these strains are affected by the external environment and their own metabolism. Easy to mutate, and become atypical strains or even die. Therefore, when preserving the strains, different media and different preservation methods should be selected for different strains, so that the preservation time of the strains is prolonged, and its main biological properties are basically unchanged, so that it can be used in the experimental teaching of microbiology . Contents of the invention [0003] Aiming at the above disadvantag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/44
Inventor 雷印平
Owner 雷印平
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