Preservation method of agricultural bacillus liquid inoculant

A technology of bacillus and liquid bacterial agent, which is applied to the preservation of agricultural bacillus liquid bacterial agent and the field of liquid bacterial agent preservation, which can solve the problem of bacterial mortality, unsatisfactory shelf life, reduced number of viable bacteria, and high production costs. problem, to achieve the effect of reducing bacterial mortality, easy operation and low cost

Pending Publication Date: 2021-10-01
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic and foreign agricultural microbial inoculum market is dominated by solid inoculum. However, compared with solid inoculum, liquid inoculum has the advantages of simple preparation process, lower production cost, and more convenient use, and has great application prospects. However, during the storage, transportation and sales of liquid bacterial agents at normal temperature, the number of viable bacteria in the bacterial agent is rapidly reduced and the shelf life is short due to unfavorable environmental conditions such as nutrient deficiencies, temperature stress, and low oxygen. Stored for 2 months, the bacterium mortality rate is as high as 31%, which seriously affects its application in agricultural production
[0004] The Chinese patent applications with application numbers 201410551199.7, 201510617246.8 and 201710641676.2 all provide methods for improving the stability and preservation effect of liquid bacterial agents, but the above methods are complicated to operate, high in production cost, and the mortality rate and shelf life of the bacteria in the preservation process are not ideal. There is a lot of room for improvement

Method used

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  • Preservation method of agricultural bacillus liquid inoculant
  • Preservation method of agricultural bacillus liquid inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 A kind of preservation method of agricultural bacillus liquid bacterial agent

[0025] S1. Preparation of bacillus liquid bacterial agent:

[0026] ① Strains

[0027] Bacillus subtilis subsp.subtilis has been deposited in the China General Microorganism Culture Collection and Management Center with the deposit number: CGMCC 1.3358, which is available to the public and has been disclosed in the patent document with the application number 201910454183.7.

[0028] ②Fermentation culture

[0029] Seed medium: glucose 20.0g / L, yeast extract powder 5.0g / L, peptone 10.0g / L, potassium dihydrogen phosphate 4.5g / L, ammonium sulfate 2.5g / L, calcium carbonate 2.0g / L.

[0030] Fermentation medium: bran 20.0g / L, peptone 5.0g / L, manganese sulfate 0.3g / L, calcium carbonate 3.0g / L, pH 8.0.

[0031] Pick 3 rings of slant-preserved strains and inoculate them into a 25L culture bottle filled with 10L seed medium, and shake and culture at 30°C and 200r / min for 14h to obtain t...

Embodiment 2-6

[0046] The preservation method of embodiment 2-6 agricultural bacillus liquid bacterial agent

[0047] Embodiment 2-6 is respectively a kind of preservation method of agricultural Bacillus liquid bacterial agent, and their steps are basically the same as embodiment 1, and the only difference is that the process parameters and detection results are different, and other parameters are the same, and the specific process is different The parameters are detailed in Table 1:

[0048] Table 1 embodiment 2-6 the storage method process parameter list of agricultural bacillus liquid inoculum

[0049]

[0050] After testing, after 12 months, the death rate of each embodiment thalline is not higher than 18.5%, shows that adopting the preservation method of the agricultural bacillus liquid bacterial agent of the present invention can effectively ensure the low death rate of the bacillus thalline, which helps Popularization and application of Bacillus liquid inoculum for agricultural us...

Embodiment 7

[0051] Example 7 The impact experiment of the pH value of bacillus liquid bacterial agent on preservation effect

[0052] Get 30mL and divide into 6 groups equally after adopting the method for embodiment 1 to make bacillus liquid bacterial agent, every group 5mL, record viable count;

[0053]Randomly select 5 groups, and use 0.1mol / L sodium hydroxide solution to treat each group separately, so that the pH value of the bacillus liquid bacterial agent is 4, 5, 6, 7 and 8 respectively;

[0054] Group 1 that was not treated with 0.1mol / L sodium hydroxide solution was used as the control group, and the pH value was 6.3 after testing;

[0055] After the above treatment, the 6 groups of Bacillus liquid bacterial agents were sealed and stored at 20°C in the dark for 60 days. On the 61st day, samples were taken to detect the number of viable bacteria, and the survival rate of the bacteria was calculated. The results are as follows: figure 1 shown.

[0056] When the pH value of the b...

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Abstract

The invention discloses a preservation method of an agricultural bacillus liquid inoculant, the method comprises the following steps: adjusting the pH value of the bacillus liquid inoculant to be neutral, adding a protective agent, sealing, and storing at room temperature, wherein a protective agent consisting of 0.8-1 part by weight of potassium sorbate, 0.5-0.7 part by weight of glycerol, 2-3 parts by weight of glucose and 3-5 parts by weight of monopotassium phosphate is added into each volume part of the agricultural bacillus liquid fungicide, and the corresponding relationship between the volume and the weight is g and L. By limiting the spore formation rate and the pH value of the bacillus liquid inoculant and adjusting the proportion of the protective agent, the protective agent is used for preserving the agricultural bacillus liquid inoculant, the preservation effect is remarkably improved, the death rate of thalli is not higher than 18.5%, and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of storage of liquid bacterial agents, and relates to a storage method for liquid bacterial agents, in particular to a method for preserving liquid bacterial agents of agricultural bacillus. Background technique [0002] Agricultural microbial agent refers to a kind of live bacterial preparation processed by the target functional bacteria after fermentation and multiplication. The application of agricultural microbial agents can enhance soil biological activity, improve soil structure, enhance soil water and fertilizer retention capacity, reduce soil-borne diseases, promote crop growth, and improve crop yield and quality. The agricultural microbial agent can be directly used as microbial fertilizer, and can also be used in the production of bio-organic fertilizer. Bacillus is an important class of biological control bacteria. It has the characteristics of fast reproduction, strong stress resistance and long...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/07
CPCC12N1/04
Inventor 赵国群白敬
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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