Strain preserving method by slope low-temperature puncture

A strain preservation and low temperature technology, applied in the preservation of microorganisms and other directions, can solve the problems of bacterial death, inability to develop, morphology, colony, and virulence easily mutated, etc., and achieve the effect of convenient use and simple operation.

Inactive Publication Date: 2017-05-03
雷印平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Bacteria are an indispensable experimental material in the teaching of microbiology experiments, and microbiology experiments cannot be carried out without it
Therefore, the laboratory has preserved various strains commonly used in teaching for a long time, but these strains are affected by the external environment and their own metabolism. Easy to mutate and become atypical strains or even die

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0010] Inoculate the pure Staphylococcus bacteria on an ordinary agar slant medium, culture it at 37 degrees for 18 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

Embodiment 2

[0012] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0013] Inoculate the purified fungus to be stored on the slant medium of Sabao weak, cultivate it at 37 degrees for 24 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

Embodiment 3

[0015] A semi-solid agar puncture strain preservation method, comprising the following steps:

[0016] Inoculate the pure hemophilic bacteria to be stored on the blood agar slant medium, culture it at 37 degrees for 21 hours, seal it with a parafilm, and store it in a refrigerator at 4 degrees.

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PUM

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Abstract

The invention discloses a strain preserving method by slope low-temperature puncture. The method comprises the steps: inoculating separated pure to-be-preserved bacteria in a proper solid slope culture medium; performing culture 18-24 hours at 37 DEG C; sealing the culture medium with a sealing film; and preserving the same in a 4 DEG C refrigerator. The method disclosed by the invention is simple to operate, convenient to use, free of special equipment and suitable for preserving strains in a lab.

Description

technical field [0001] The invention relates to the field of strain preservation, in particular to a low-temperature strain preservation method on an inclined plane. Background technique [0002] Bacteria is an indispensable experimental material in the teaching of microbiology experiments, and microbiology experiments cannot be carried out without it. Therefore, the laboratory has preserved various strains commonly used in teaching for a long time, but these strains are affected by the external environment and their own metabolism. Easy to mutate, and become atypical strains or even die. Therefore, when preserving the strains, different media and different preservation methods should be selected for different strains, so that the preservation time of the strains is prolonged, and its main biological properties are basically unchanged, so that it can be used in the experimental teaching of microbiology . Contents of the invention [0003] Aiming at the above disadvantag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
Inventor 雷印平
Owner 雷印平
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