Vaccinium tissue rapid propagation medium and its cultivation method

A medium and inducing medium technology, applied in the field of plant tissue culture, can solve the problems of poor in vitro rapid propagation effect and the like, and achieve the effect of shortening the process of popularization of improved varieties

Inactive Publication Date: 2017-09-05
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the existing medium has poor effect on the in vitro rapid propagation of lingonberry tissue, and provides a rapid propagation medium for lingonberry tissue and a cultivation method thereof

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0032] Specific embodiment 1: In this embodiment, the rapid propagation medium of European lingonberry tissue includes two kinds of culture medium: axillary bud induction medium and differentiation and proliferation medium; wherein, the described axillary bud induction medium contains 0.1mg / L~1.0mg / L Dy medium of L zeatin;

[0033] The differentiation and proliferation medium is Dy medium containing 0.1mg / L~1.0mg / L 6-benzylaminopurine and 0.1mg / L~1.0mg / L zeatin;

[0034] Wherein, the Dy medium is composed of macroelements, calcium salts, trace elements, iron complex salts, organic substances, and the strength is 1400g / cm 2 of agar and distilled water;

[0035] Each 1L of Dy medium contains the above-mentioned macroelements ranging from 180mg / L to 200mg / L of KNO 3 , 400mg / L~410mg / L NH 4 NO 3 , 365mg / L~380mg / L of MgSO 4 ·7H 2 O and 160mg / L~180mg / L of KH 2 PO 4 composition;

[0036] Every 1L of Dy medium contains said calcium salt as Ca(NO) of 680mg / L~690mg / L 3 ) 2 4H ...

specific Embodiment approach 2

[0041] Embodiment 2: This embodiment differs from Embodiment 1 in that: the axillary bud induction medium is Dy medium containing 0.6 mg / L zeatin. Others are the same as the first embodiment.

specific Embodiment approach 3

[0042] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: the differentiation and proliferation medium is Dy medium containing 0.5 mg / L 6-benzylaminopurine and 1.0 mg / L zeatin . Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to the field of plant tissue culture, in particular to rapid propagation culture media for vaccinium myrtillus tissue and a cultivation method of the vaccinium myrtillus tissue, and aims to solve the problem of poor effect of a conventional culture medium on rapid in-vitro propagation of the vaccinium myrtillus tissue. The rapid propagation culture media for the vaccinium myrtillus tissue comprise an axillary bud induction culture medium and a differentiation and proliferation culture medium, wherein the axillary bud induction culture medium is a Dy culture medium containing zeatin, and the differentiation and proliferation culture medium is a Dy culture medium containing 6-benzyl aminopurine and zeatin. The method comprises steps as follows: step one, material selection and sterilization; step two, axillary bud culture as well as differentiation and proliferation. The rapid propagation culture media for the vaccinium myrtillus tissue and the cultivation method of the vaccinium myrtillus tissue are applied to rapid propagation and culture for the vaccinium myrtillus tissue.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a culture medium for rapid propagation of bilberry tissue and a cultivation method thereof. Background technique [0002] European lingonberry (Vaccinium myrtillus L.) is a perennial berry growing in Central Europe, mainly distributed in Sweden, Finland, Ukraine, northern Russia, Latvia and other central and northern European alpine regions. It is also distributed in the Altai Mountains of Xinjiang, my country. It has strong cold resistance and can withstand extreme low temperatures of -50°C. Due to the unique geographical environment and climatic factors, the types and contents of anthocyanins in the fruit of European lingonberry are higher than those of other lingonberry varieties. European lingonberry extract, European lingonberry fresh fruit, jams and juices are very popular in the market. The price of fresh fruit in the European market is 5 Euros / kg, and the price of jam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 田新华姜天瑞翁海龙殷东生刘明辉王福德张建瑛温爱亭李正华
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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