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A sample-carrying copper grid fixing device

A fixing device and copper mesh technology, which is applied in the preparation of test samples and other directions, can solve the problems of easy pinching of Fanghua film, affecting observation, and difficult to unify the dyeing time, and achieve the effect of automatic dyeing and saving dye liquor.

Active Publication Date: 2019-05-14
河南中镜科仪科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] When staining, first use tweezers to clamp the 0.4mm ring width at the edge of the copper grid. If the tweezers are clamped to the grid area of ​​the copper grid, it is easy to pinch the Fanghua membrane attached to the grid surface, and then destroy the cell slices carried on the copper grid. , impact observation
Usually, the amount of sections processed each time is relatively large (4-20 pieces, or even more). If each copper mesh is dyed one by one, the dyeing time of each section is not easy to be uniform; when switching between different staining steps, rely on tweezers Repeated clamping of each copper mesh is likely to cause mechanical damage to the slice and affect the observation

Method used

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  • A sample-carrying copper grid fixing device
  • A sample-carrying copper grid fixing device
  • A sample-carrying copper grid fixing device

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Embodiment Construction

[0028] The fixing device of the present invention will be further described in detail below with reference to the accompanying drawings.

[0029] figure 1 It is a schematic diagram of an arc-shaped fixing device. The fixing device includes a main body 1 and an auxiliary body 2. The auxiliary body 2 plays a role in fixing and supporting the main body 1 of the net. The main body 1 of the net is made of a hard layer of plastic, glass, and stainless steel. 101. The soft layer 102 of wax is composed of a hard layer 101 of plastic, glass, and stainless steel covered with a layer of wax layer 102, and the wax layer 102 at the opening of the arc is symmetrical to the two ends of the copper mesh 103 For fixing, the distance between the wax layer 102 arc-shaped openings is 1.2mm to 2.8mm, the thickness of the wax layer is 0.4mm to 1.5mm, and the distance between the arc-shaped openings of the hard layers of the plastic layer, glass layer, and stainless steel layer The distance is 1.6mm...

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Abstract

The invention relates to a fixing device for a sample-loading copper grid. The fixing device comprises a loading grid main body and an auxiliary body, wherein the loading grid main body is the part for fixing the sample-loading copper grid; the auxiliary body is the part for fixing and supporting the sample-loading copper grid; the loading grid main body is composed of a hard layer and a soft layer; the soft layer covers the hard layer; the flexibility and fixing property of the soft layer are utilized to fix the copper grid in the fixing device. The hard layer is made of glass, stainless steel and plastics. The soft layer is made of wax.

Description

technical field [0001] The invention relates to a carrier for fixing a sample-carrying copper net for a biological transmission electron microscope, in particular to a device for high-throughput fixing the sample-carrying copper net in the sample dyeing link. Background technique [0002] The copper grid is the tool for carrying the sample in the transmission electron microscope. It consists of two parts, the edge is a copper ring with an outer diameter of 3mm, an inner diameter of 2.6mm, and a ring width of about 0.4mm; within the ring width there are hollow grids ranging from 50 to 400 meshes; the thickness of the entire copper mesh is about 40μm. Usually one side of the copper mesh is covered with a layer of organic Fanghua film, which can effectively support the sliced ​​sample, which is called the front side. Once this layer of Fanghua membrane breaks or falls off, the biological sample also breaks or falls off. Staining of biological samples is carried out on copper ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 梁静南梁好刘一苇孙树涛赵彤杨琳张建国
Owner 河南中镜科仪科技有限公司
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