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Cell culture medium and culture method using same

A technology of cell culture and culture method, which is applied in the field of evaluation of culture medium for culturing cells, and can solve problems such as no report

Active Publication Date: 2016-09-21
KANEKA CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the addition of GAS6 to serum-free media

Method used

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  • Cell culture medium and culture method using same
  • Cell culture medium and culture method using same
  • Cell culture medium and culture method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0150] (1) Detection of proteins in domesticated serum-free medium

[0151] Acclimatization medium (CM) was prepared from serum-free medium using mouse embryonic fibroblasts (MEF cells) not activated by mitomycin treatment. Mouse embryonic fibroblasts (MEF cells) that were not activated by mitomycin treatment were seeded in a medium for MEF (DMEM medium supplemented with 10% FBS) at a cell density of about 500,000 cells / dish of 60 mm in diameter . After culturing the cells for at least 16 hours, wash with PBS (-), then wash with Essential8 medium (Invitrogen) (hereinafter, this serum-free medium will also be referred to as E8 medium), and replace the medium with the same serum-free medium.

[0152] After replacing the medium, the CO 2 Cultured in an incubator (37°C, 5% CO 2 concentration) for 24 hours, and then the culture medium was recovered to obtain the acclimatization medium.

[0153] The non-acclimated serum-free medium and the acclimatized serum-free medium were co...

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PUM

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Abstract

The purpose of the present invention is to provide a cell culture medium, particularly a serum-free cell culture medium, that makes it possible to raise cell growth efficiency without using feeder cells. The present invention provides a serum-free cell culture medium containing growth arrest-specific 6 (GAS6).

Description

technical field [0001] The invention relates to a cell culture medium and a method for culturing cells. Furthermore, the present invention also relates to a feeder cell that can be used in the above-mentioned culture method, and a method for evaluating a medium for culturing the cells. Background technique [0002] Recent studies have shown that human pluripotent stem cells such as human ES cells (hESCs) and human iPS cells (hiPSCs) are likely to be practically used in the field of regenerative medicine. These pluripotent stem cells have the ability to proliferate indefinitely and differentiate into various cells, and are expected to be used as therapeutic methods for intractable diseases and lifestyle-related diseases. Human pluripotent stem cells have been shown to be induced to differentiate into various cells such as nerve cells, cardiomyocytes, blood cells or omental cells in vitro. [0003] The feeder cells serve to supply the stem cells with growth factors for maint...

Claims

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Application Information

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IPC IPC(8): C12N5/10C07K14/47
CPCC07K14/47C12N2501/998C12N5/0037C12N5/0696C12N2500/90C12N2533/90
Inventor 加藤智久金村米博正札智子福角勇人
Owner KANEKA CORP
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