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11-Dehydro-thromboxane B2 determination kit and use thereof

A kit and thromboxane technology, which is applied in the field of 11-dehydro-thromboxane B2 determination kits, can solve the problems of large manpower, material resources and time, difficulty in batch automatic detection, low precision and the like, and achieve accuracy High, high sensitivity, good precision effect

Active Publication Date: 2016-09-28
SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method takes about 4 hours, and the precision is low
[0011] The above two methods to detect aspirin resistance require a lot of manpower, material resources and time, and the cost is high, so it is difficult to realize batch automatic detection

Method used

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  • 11-Dehydro-thromboxane B2 determination kit and use thereof
  • 11-Dehydro-thromboxane B2 determination kit and use thereof
  • 11-Dehydro-thromboxane B2 determination kit and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of 11-dehydro-thromboxane B2 assay kit

[0034] 1. Preparation of reagent R1:

[0035] Weigh 0.015 mg of the 11dhTxB2 monoclonal antibody and dissolve it in 100 ml of 10 mmol / L Tris buffer to dissolve completely. After filtering through a 0.22 μm filter membrane, a 0.015% (W / V) reagent R1 is prepared.

[0036] 2. Preparation of reagent R2:

[0037] Use 30nm hydroxylated polystyrene nano-latex particles, add EADC:Sulfo-NHS:nano-latex particles to the MES buffer with pH=6.0, the mass ratio is 1:0.5:0.1, ultrasonic 2-3 seconds, mix well , activated on a rotary shaker at room temperature for 2 hours, centrifuged at 17000rpm for 30 minutes, washed 3 times with MES buffer solution with pH = 6.0, then suspended the microspheres in HEPES buffer solution with pH = 8.0 to make microparticle suspension, and measured particle size diameter and CV value.

[0038] Add 11dhTxB2 protein whose mass is 1 / 3 of the weight of nano-latex particles to the 30nm microp...

Embodiment 2

[0043] Example 2: Preparation of 11-dehydro-thromboxane B2 assay kit

[0044] The difference from Example 1 is that 2% (W / V) of reagent R1 and 2% (W / V) of reagent R2 are prepared, wherein 400nm hydroxylated acrylate nano latex particles are used in the preparation of reagent R2.

Embodiment 3

[0045] Example 3: Preparation of 11-dehydro-thromboxane B2 assay kit

[0046] The difference from Example 1 is that 1% (W / V) of reagent R1 and 1% (W / V) of reagent R2 are made, wherein 200nm of hydroxylated divinylbenzene nano latex particles are used in the preparation of reagent R2 .

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Abstract

The invention discloses a 11-dehydro-thromboxane B2 determination kit. The kit comprises: a reagent R1 including 0.01-2% (w / v) of 11dhTxB2 antibody and a buffering solution; a reagent R2 including 0.1-2% (w / v) of a 11dhTxB2 protein coated latex nanoparticle buffering solution; a calibrating substance comprising the 11dhTxB2 protein and a buffering solution; and a quality control substance which is a 11dhTxB2 protein dissolved in artificial urine. The 11-dehydro-thromboxane B2 determination kit improves the sensitivity and the linear range of detection of the 11-dehydro-thromboxane B2 content of urine, the detection sensitivity can reach 775pg / ml, and the linear range can reach 0-6300pg / ml. The 11-dehydro-thromboxane B2 determination kit has the advantages of high sensitivity, good specificity, high accuracy, good precision and good stability.

Description

technical field [0001] The invention relates to a detection kit, in particular to a 11-dehydro-thromboxane B2 detection kit, and also relates to the application of the kit. Background technique [0002] Due to the antiplatelet effect of aspirin, high-risk patients can reduce the incidence of arterial thrombotic events by about 25%. However, in patients with arterial thrombosis who received aspirin therapy, 10% to 25% of patients with arterial thrombosis will relapse during long-term follow-up. This phenomenon is called aspirin resistance or drug resistance. The emergence of this point of view has made people question the effectiveness of aspirin, because after taking aspirin, the platelet function of quite a few patients is still normal. [0003] Hungarian scholar Pusch et al. conducted a systematic review of the relevant data on the incidence of aspirin resistance and its clinical relationship before 2008. They found that although there is currently no widely accepted sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/539
CPCG01N33/539G01N33/54346
Inventor 张立民王琳王元略李丰骥
Owner SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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