Marine Streptomyces sp. and application of Streptomyces sp. in preparation of nosiheptide

A technology of marine streptomyces and nosiheptide, which is applied in the field of microorganisms, can solve the problems such as the decrease in the number of new antibacterial antibiotics found, the inability to meet the needs of infectious disease prevention and control, and achieve the effect of broad application prospects.

Active Publication Date: 2016-11-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In stark contrast to this, the number of discoveries of new antibacterial antibiotics has dropped sharply. In the past 10 years, only a few new antibiotics such as daptomycin and tigecycline have been on the market, which is far from meeting the needs of human beings for the prevention and treatment of infectious diseases.

Method used

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  • Marine Streptomyces sp. and application of Streptomyces sp. in preparation of nosiheptide
  • Marine Streptomyces sp. and application of Streptomyces sp. in preparation of nosiheptide
  • Marine Streptomyces sp. and application of Streptomyces sp. in preparation of nosiheptide

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Embodiment 1

[0018] Embodiment 1: Isolation and identification of marine streptomyces (Streptomyces sp.) SCSIO 1682

[0019] The marine streptomyces (Streptomyces sp.) SCSIO 1682 of the present invention is isolated from a sediment sample at a water depth of 45m in the Luhuitou sea area of ​​Sanya, my country (109°48'E, 18°23'N).

[0020] The taxonomic characteristics of this strain are:

[0021] 1. Morphological characteristics:

[0022] The colony is dry, and the single colony is generally round, small, compact, difficult to spread, and raised in the middle. The hyphae in the base and the aerial hyphae are tightly combined and difficult to stir up. After the final spore production, the spores are easy to scrape. Light brown aerial hyphae and yellow stroma hyphae formed on ISP4 medium.

[0023] 2. Molecular biology separation characteristics:

[0024] Extract the genomic DNA of the above-mentioned isolated bacterial strain, amplify its 16S rDNA sequence by conventional method PCR, and ...

Embodiment 2

[0026] Embodiment 2: Separation and identification of Nosiheptide

[0027] 1. Prepare the fermentation culture of Streptomyces sp. SCSIO 1682:

[0028] (1) Preparation of seed medium and fermentation medium:

[0029] a) Preparation of seed medium: every liter of seed medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix the above components evenly, and adjust the pH to 7.0. Prepare 1L of seed medium, and then equally distribute it in 20 250mL Erlenmeyer flasks, each containing 50mL of seed medium, and sterilize at 121°C for 30min for later use.

[0030] b) Preparation of fermentation medium: every liter of fermentation medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix...

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Abstract

The invention discloses marine Streptomyces sp. and an application of Streptomyces sp. in preparation of nosiheptide. The marine Streptomyces sp. SCSIO 1682 is preserved in the CCTCC (China Center for Type Culture Collection) in Wuhan University, Wuhan City, China on June 6, 2016 and has the preservation number being CCTCC NO: M2016307. A fermentation culture of the marine Streptomyces sp. SCSIO 1682 can be used for preparing nosiheptide shown in the formula (I). A biological preparation method is provided for production and preparation of nosiheptide, and the marine Streptomyces sp. has broad application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a marine streptomyces (Streptomyces sp.) SCSIO 1682 and its application in preparing nosiheptide. Background technique: [0002] In recent years, the uncontrolled addition of antibiotics in the field of animal breeding and the long-term, repeated, and high-dose abuse of antibiotics in the clinical field have exacerbated the rapid emergence and spread of various types of drug-resistant pathogens, such as methicillin-resistant Staphylococcus aureus (methicillin-resistant Staphylococcus aureus, MRSA), vancomycin-resistant Enterococcus (VRE), multi-drug-resistant tuberculosis (multidrug-resistant tuberculosis, MDRMT). "NDM-1 producing resistant bacteria (New Delhi-metallo-β-lactamase, New Delhi metallo-β-lactamase)" of "Superbugs". In stark contrast to this, the number of new antibacterial antibiotics has dropped sharply. In the past 10 years, only a few new anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/02C12R1/465
CPCC12P21/02C12N1/205C12R2001/465
Inventor 鞠建华桂春李青连程伟鸽
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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