Marine Streptomyces sp. and application of Streptomyces sp. in preparation of nosiheptide
A technology of marine streptomyces and nosiheptide, which is applied in the field of microorganisms, can solve the problems such as the decrease in the number of new antibacterial antibiotics found, the inability to meet the needs of infectious disease prevention and control, and achieve the effect of broad application prospects.
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Embodiment 1
[0018] Embodiment 1: Isolation and identification of marine streptomyces (Streptomyces sp.) SCSIO 1682
[0019] The marine streptomyces (Streptomyces sp.) SCSIO 1682 of the present invention is isolated from a sediment sample at a water depth of 45m in the Luhuitou sea area of Sanya, my country (109°48'E, 18°23'N).
[0020] The taxonomic characteristics of this strain are:
[0021] 1. Morphological characteristics:
[0022] The colony is dry, and the single colony is generally round, small, compact, difficult to spread, and raised in the middle. The hyphae in the base and the aerial hyphae are tightly combined and difficult to stir up. After the final spore production, the spores are easy to scrape. Light brown aerial hyphae and yellow stroma hyphae formed on ISP4 medium.
[0023] 2. Molecular biology separation characteristics:
[0024] Extract the genomic DNA of the above-mentioned isolated bacterial strain, amplify its 16S rDNA sequence by conventional method PCR, and ...
Embodiment 2
[0026] Embodiment 2: Separation and identification of Nosiheptide
[0027] 1. Prepare the fermentation culture of Streptomyces sp. SCSIO 1682:
[0028] (1) Preparation of seed medium and fermentation medium:
[0029] a) Preparation of seed medium: every liter of seed medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix the above components evenly, and adjust the pH to 7.0. Prepare 1L of seed medium, and then equally distribute it in 20 250mL Erlenmeyer flasks, each containing 50mL of seed medium, and sterilize at 121°C for 30min for later use.
[0030] b) Preparation of fermentation medium: every liter of fermentation medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix...
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