Method for acquiring broccoli microspore material in winter
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microspores and broccoli, which is applied in the fields of botany equipment and methods, plant cells, soilless cultivation, etc., can solve the technical difficulties of obtaining broccoli microspores, failure to extract microspore flower buds normally, and cultivation and breeding of broccoli microspores And other issues
Active Publication Date: 2017-01-04
ZHEJIANG MITSUO SEED CO LTD
View PDF10 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] Moreover, in the normal flowering season of broccoli, a large amount of microspores can be obtained; but when the suitable flowering season is over, after entering autumn and winter, due to the unfavorable external cultivation conditions, flower buds suitable for microspores cannot be normally drawn out, and then cannot be produced in the broccoli. Obtain broccoli microspores in winter to provide resources for creating DH (double haploid) materials, which brings more difficulties to broccoli microspore cultivation and breeding technology
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0033] A kind of method that obtains broccoli microspore material in winter, described method mainly comprises the following steps:
[0034] a. Sowing: In the greenhouse, prepare the hole tray, fill the cultivation substrate in the hole tray, and pour water thoroughly, sow the broccoli seeds shallowly in the hole tray, such as figure 1 shown;
[0035] b. Accelerating germination: Place the above-mentioned hole trays sown with broccoli seeds on a hotbed for accelerating germination. The germination temperature is controlled at 20-25°C during the day and 10-15°C at night; during the germination period, the watering management is as follows: figure 2 shown;
[0036] c. Colonization: When 2 to 3 true leaves grow, plant them in the matrix nutrition cup, and regularly water the nutrient solution MB1 according to the dryness and humidity of the matrix, and control the environmental temperature and humidity, so as to meet the requirements of its vegetative growth. required conditio...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
length
aaaaa
aaaaa
length
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a method for acquiring a broccoli microspore material in winter. The method comprises the following steps: a, sowing seeds, namely, sowing seeds of broccoli into a shallow layer of a hole tray; b, accelerating germination, namely, putting the hole tray on a hotbed for accelerating germination at 20-25 DEG C at daytime and 10-15 DEG C at night; c, performing fixed planting, namely, when 2-3 true leaves grow out, performing fixed planting and cultivation in medium nutrient cups, and irrigating with a nutrient solution MB1 at regular time; d, performing low-temperature vernalization, namely, after 14-16 true leaves grow out, performing low-temperature vernalization for about 20 days at 8-15 DEG C, and irrigating with a nutrient solution MB2; e, cultivating, namely, after ball-flowers are formed, irrigating with a nutrient solution MB3, cultivating continuously, and controlling the ambient temperature inside a greenhouse to be 20-25 DEG C so as to obtain normal flower buds; f, extracting microspores, namely, sterilizing selected flower buds, and extracting pollen, thereby obtaining the microspore material. By adoption of the soilless cultivation technique, the flower buds applicable to extraction of the microspore material can be cultivated in the greenhouse in winter, and the technical difficulty that broccoli microspores are hard to obtain normally in winter can be solved.
Description
technical field [0001] The invention relates to a method for obtaining microspore materials, more particularly to a method for obtaining broccoli microspore materials in winter. Background technique [0002] Broccoli, also known as broccoli, green cauliflower, stem broccoli, etc., is a primary or secondary herbaceous plant in the Brassicaceae Brassica species with green curds as the product organ. As a product, the curd is rich in nutrition, good in color, aroma and taste, and is a famous and special vegetable that is very popular in the international market. [0003] Since Lichter reported for the first time in 1982 that rapeseed free microspore culture obtained regenerated plants, Brassica crops, especially the microspore culture of rapeseed, have achieved remarkable results in terms of embryo emergence rate, embryo yield, embryo culture and regenerated plants. Great success. Compared with Brassica napus, head cabbage and other crops, the cultivation of free microspores ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.