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A kind of establishment method of gossypol-induced fish enteritis model

A technique for establishing a method and inducing fish, which is applied in fish farming, animal husbandry, and material inspection products, can solve the problems of fish death, high mortality, and application limitations of fish enteritis models, and achieve good reproducibility and model Stable and reliable effect

Active Publication Date: 2019-03-19
SICHUAN AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In many cases, if a fish enteritis model established by an inappropriate method is used, it will easily lead to too severe lesions of fish enteritis, leading to fish death and high mortality, and the instability of the fish enteritis model, which limits the application of the fish enteritis model

Method used

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  • A kind of establishment method of gossypol-induced fish enteritis model

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Embodiment l-6

[0027] Embodiment 1-6: the establishment method of a kind of gossypol-induced fish enteritis model, comprises the following steps:

[0028] 1. According to the conventional method, 540 mid-growth grass carp of 230-727g were used as the objects for establishing the enteritis model, and were randomly divided into 6 treatment groups, with 3 repetitions in each group and 30 fish in each repetition.

[0029] 2. Preparation of experimental feed containing gossypol

[0030] Mix gossypol evenly in the feed whose main components are fish meal, soybean protein concentrate, rice protein powder, cornstarch and α-starch, and prepare gossypol content of 0, 125, 250, 375, 775 and 1175mg / kg respectively The experimental feed was pelletized and stored in a 4°C refrigerator for later use;

[0031] 3. Model establishment

[0032] The grass carp selected in step 1 was raised in a cage of 1 cubic meter, and the experimental feed prepared in the above step 2 was fed with 3 wt% of the fish body we...

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Abstract

The invention discloses a method for establishing a gossypol-induced fish enteritis model, which comprises the following steps: selecting normal cultured fish as the object for establishing the enteritis model; taking experimental feed with a gossypol content of 250 mg / kg, and feeding the above-mentioned cultured fish, A fish enteritis model induced by gossypol was obtained. The establishment method of the fish enteritis model induced by gossypol in the present invention has the least impact on the production performance, intestinal growth and development, immune ability and muscle quality of fish, but the pathological manifestation of enteritis caused by it is the most suitable, the model is stable, reliable and reproducible it is good.

Description

technical field [0001] The invention relates to a method for establishing an animal model, in particular to a method for establishing a gossypol-induced fish enteritis model. Background technique [0002] Cottonseed meal is a flour cake obtained after cottonseed is pressed, and most of the residual oil in it is separated through leaching process to obtain a reddish or yellow granular product, which is the main raw material for processing and manufacturing feed. The crude protein contained can reach 40%, even as high as 44%, which is comparable to the crude protein content of soybean meal; at the same time, the price of cotton meal is lower than that of soybean meal, and the source is extensive. The output of cotton meal in my country is high, with an annual output of 12 million tons above. Therefore, due to the special physiological characteristics of fish, cotton meal has become an important high-quality and cheap protein feed source for fish feed. However, gossypol contain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13
CPCA01K61/00G01N33/50A01K2207/20A01K2227/40A01K2267/0368A01K67/027
Inventor 周小秋王开卓冯琳姜维丹刘扬吴培姜俊唐旭
Owner SICHUAN AGRI UNIV
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