A kind of red Ganoderma lucidum extract reference substance and its preparation method and application

A technology of Ganoderma lucidum extract and red Ganoderma lucidum, applied in the field of red Ganoderma lucidum reference substance extract and its preparation, can solve the problems of inability to fully and accurately evaluate the quality of red Ganoderma lucidum, high cost, etc., to save time and cost of detection, and good stability , Simple and convenient to use

Active Publication Date: 2019-03-12
GUANGZHOU SHENNONG BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to make up for the defects in the prior art, the present invention provides a red ganoderma extract reference substance and a preparation method of the red ganoderma lucidum extract reference substance, which can effectively solve the high cost of the reference substance in the quality evaluation of red ganoderma lucidum, and cannot be comprehensive and accurate Questions about evaluating the quality of red Ganoderma lucidum

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  • A kind of red Ganoderma lucidum extract reference substance and its preparation method and application
  • A kind of red Ganoderma lucidum extract reference substance and its preparation method and application
  • A kind of red Ganoderma lucidum extract reference substance and its preparation method and application

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preparation example Construction

[0048] The preparation of the red ganoderma lucidum extract is preferably carried out according to a method comprising the following steps: pulverizing the red ganoderma lucidum, adding a solvent for extraction, filtering the extract, and collecting the filtrate. Preferably, the extraction adopts heating reflux extraction, ultrasonic extraction, microwave extraction, percolation extraction or flash extraction, more preferably, the extraction adopts heating reflux extraction or ultrasonic extraction. Preferably, the step of concentrating the filtrate under reduced pressure is also included; further preferably, the step of adding auxiliary materials to the concentrate and continuing to concentrate under reduced pressure to dryness is also included. Further preferably, the auxiliary material is at least one of dextrin, micropowder silica gel, starch, carboxymethyl cellulose, more preferably, the auxiliary material adopts micropowder silica gel, because micropowder silica gel is in...

Embodiment 1

[0060] The establishment of the red ganoderma lucidum standard collection of illustrative plates of embodiment 1

[0061] 1.1. Experiment overview:

[0062] The collected 50 kinds of Ganoderma lucidum medicinal materials (numbered 1-50) from different origins were identified by TLC, and the fluorescent spots of the same color were screened out at the positions corresponding to the TLC and red Ganoderma lucidum reference medicinal materials. Ganoderma lucidum medicinal materials, a total of 20 kinds. The 20 kinds of red ganoderma lucidum were analyzed by liquid chromatography, and the resulting spectra were input into the Chinese medicine chromatographic fingerprint similarity evaluation system (version 2012.1) to generate the red ganoderma standard spectrum (see Figure 7 )

[0063] 1.2 TLC identification of 50 kinds of Ganoderma lucidum medicinal materials

[0064] 1.2.1. Sample preparation of 50 kinds of ganoderma lucidum

[0065] Weigh 2g of Ganoderma lucidum, add 30ml ...

Embodiment 2

[0082] Embodiment 2 is used for the optimization of the red ganoderma lucidum raw material that red ganoderma extract reference substance prepares

[0083] 2.1. Experiment overview

[0084] The raw materials of red ganoderma lucidum used in the preparation of red ganoderma extract reference substance shall meet the following quality standards:

[0085] (1) The medicinal material is analyzed by liquid chromatography and compared with the standard spectrum of red ganoderma lucidum, the similarity should be greater than 90% (using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system version 2012.1)

[0086] (2) The content of ganoderma acid A is more than 0.02%, the content of ganoderma acid B is more than 0.01%, the content of ganoderma acid C2 is more than 0.015%, and the content of ganoderma acid G is more than 0.015%.

[0087] According to the above quality standards, the raw medicinal materials were optimized from the 6 kinds of red gano...

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Abstract

The invention provides a ganoderma lucidum extract reference substance and a preparation method and an application thereof. The preparation method of the ganoderma lucidum extract reference substance includes the step of extracting ganoderma lucidum to obtain ganoderma lucidum extracts and also includes the step of blending the different ganoderma lucidum extracts with each other to obtain the ganoderma lucidum extract reference substance; the ganoderma lucidum extract reference substance contains the following components by the mass percentage: 0.3%-0.5% of ganoderic acid A, 0.15%-0.4% of ganoderic acid B, 0.3%-0.5% of ganoderic acid C2, and 0.25%-0.5% of ganoderic acid G. With the ganoderma lucidum extract reference substance provided by the inveniton, the problems that the cost of a reference substance in quality evaluation of ganoderma lucidum is high and good and bad of ganoderma lucidum cannot be fully and accurately evaluated can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of ganoderma quality control, in particular to a red ganoderma reference substance extract and a preparation method and application thereof. Background technique [0002] Ganoderma lucidum has a history of thousands of years in our country. It is recorded in "Shen Nong's Materia Medica" that Ganoderma lucidum strengthens the foundation and nourishes the vitality. [0003] However, Ganoderma lucidum, as a kind of fungal medicinal material, has many varieties, and the chemical composition of different varieties of Ganoderma lucidum varies greatly. Even if it is the same red Ganoderma lucidum, it is divided into many subspecies. The silk body is also very different from the fruiting body in composition. The efficacy of traditional Chinese medicine is based on the stable composition and content of the chemical substances it contains. If the chemical composition and content vary greatly, then its efficacy will i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 李莹雪李乾坤王锐银黄静贤李紫薇
Owner GUANGZHOU SHENNONG BIOLOGICAL TECH
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