Method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers

A biomarker, kidney-yang deficiency technology, applied in biological tests, measuring devices, material testing products, etc., can solve the problem of not being able to determine kidney-yang deficiency syndrome biomarkers and other issues

Active Publication Date: 2017-03-22
王喜军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the prior art cannot determine the biomarkers of kidney-yang...

Method used

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  • Method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers
  • Method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers
  • Method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers

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Experimental program
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specific Embodiment approach 1

[0063] Specific Embodiment 1: A method for detecting, screening and identifying metabolic biomarkers of kidney-yang deficiency syndrome according to this embodiment is specifically prepared according to the following steps:

[0064] Step 1: Replicate the rat model of kidney yang deficiency; subcutaneously inject olive oil solution into each clean-grade rat in the normal group, and subcutaneously inject 10 mg / mL cortex into each clean-grade rat in the model group at 0.1 mL / Kg body weight Ketone olive oil solution; collect urine samples, blood samples and organ samples of clean rats in normal group and model group;

[0065] Step 2. Evaluate the rat model of kidney-yang deficiency; the clinical location of kidney-yang deficiency is the inhibition of the neuroendocrine immune network, and the corresponding hormone levels and the functional status of the target organs involved in the neuroendocrine immune network can reflect whether it is In a suppressed state; therefore, the clini...

specific Embodiment approach 2

[0082] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the urine samples collected from the normal group and the model group are specifically:

[0083] (1), the clean level rats are completely randomly divided into two groups, i.e. normal group and model group;

[0084] (2), each rat of normal group is injected olive oil solution subcutaneously by 0.1mL / Kg body weight, and each rat of model group is injected 10mg / mL corticosterone olive oil solution subcutaneously by 0.1mL / Kg body weight,

[0085] (3) Urine samples were collected from each rat for 12 hours, and the fresh urine samples were stored at -80°C under the conditions of centrifugation at 4°C and 13,000 rpm for 10 minutes, and thawed at room temperature before analysis. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0086] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the blood samples collected from the normal group and the model group of clean rats are specifically:

[0087] 1) Blood was collected from the abdominal aorta of each rat, and the serum was separated under the conditions of centrifugation at 4°C, 4000r / min, and 10min;

[0088] 2) Aliquoted and stored in a -80°C refrigerator, and thawed at room temperature before analysis. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a method for detecting, screening and identifying metabolism biomarkers, in particular to a method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers. In order to solve the problem that in the prior art kidney-yang deficiency metabolism biomarkers cannot be determined, the method for detecting, screening and identifying kidney-yang deficiency metabolism biomarkers comprises the steps that a kidney-yang deficiency rat model is copied, a kidney-yang deficiency rat model is evaluated, a rat urine or blood QC sample is obtained through preparation; urine endogenous metabolites and blood endogenous metabolites are obtained; 33 urine biomarkers and 17 blood biomarkers are obtained through determination and identification of a rat urine metabolism counter track variation trend PCA score chat. The method is applied to the field of metabolism biomarker detection, screening and identification.

Description

technical field [0001] The invention relates to a method for detecting, screening and identifying metabolic biomarkers of kidney-yang deficiency syndrome. Background technique [0002] The Zangxiang in Chinese medicine is "hidden in the inside, and the shape is seen outside", and clinically it is manifested in "syndrome". The essence of "syndrome" is the change of metabolism or its network caused by the imbalance of the body. This characteristic phenotype is the change of the expression profile of the endogenous metabolic components (small molecule metabolites) secreted into the blood or urine by the organs. be reflected objectively. Metabolomics is a science that studies the types, quantities and changes of metabolites (endogenous metabolites) after the body is disturbed by external factors. Therefore, the rise of metabolomics provides a method to integrate and analyze multi-level and multi-dimensional data from the system level, expressing the characteristics of traditio...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N30/02G01N30/72
CPCG01N30/02G01N30/7266G01N33/5091
Inventor 王喜军张爱华刘琦周晓航南洋
Owner 王喜军
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