Overlapped protein point separation method and device based on concave point matching

Abstract

The invention discloses an overlapped protein point separation method and device based on concave point matching, and the method comprises the steps: solving all angular points on an overlapped protein point outline boundary in a gel image through employing a Harris angular point detection method; constructing an approximate polygon of the overlapped protein point outline boundary in the gel image based on a polygonal approximation algorithm of angular points through all solved angular points, thereby judging the concave and convex points of the top points of the approximate polygon and obtaining a concave angular point; selecting a matching rule through a preset concave point, selecting concave points from the concave angular points, constructing a separation line, and completing the segmentation of overlapped protein points in the gel image. The method can improve the segmentation effect of the overlapped protein points in a bidirectional gel image.

Description

technical field [0001] The invention belongs to the technical field of two-way gel image segmentation by computer, and in particular relates to a method and device for separating overlapping protein spots based on pit point matching. Background technique [0002] Proteomics opens new horizons for many research fields in the life sciences. From the prediction of cancer drug development to the detection of environmental pollution, the application range of proteomics is wide. Proteomics analysis includes protein identification and functional determination, and the first step is protein detection and analysis. Two-dimensional gel electrophoresis (two-dimensional gel electrophoresis, 2-DE) technology is one of the more classic protein separation techniques. It can separate highly complex protein mixtures from cells or tissues, and can separate a large number of protein spots while maintaining the natural charge and solubility of protein spots. The obtained gel is scanned by a ...

AI Technical Summary

Problems solved by technology

[0008] (1) Most of the existing protein spot detection methods are only ideal for high-quality gel image detection results, but for the overlapping and accumulation of protein spots in the gel image, most of them cannot be properly handled, and often require Relying on manual experience to input some detection parameters can obtain better detection results, and the accuracy is not high

[0009] (2) Even the algorithm for the separation of overlapping targets has certain limitations for the separation of overlapping protein spots in gel images, and cannot be well applied to gel images, mainly because the protein spots have different shapes and are not strict. The circular structure, and the overlapping situation is generally more complicated, the degree of adhesion is tight, and there is no obvious depression or grayscale difference

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