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Method for detecting influence of electronic cigarette product on cell active oxygen secretion

A cell activity, electronic cigarette technology, applied in material testing products, analytical materials, biological testing, etc., can solve the problems of time-consuming and labor-intensive, no unified standard method, etc., and achieve the effect of optimizing the detection dose

Active Publication Date: 2017-04-05
CHINA TOBACCO YUNNAN IND
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Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological test indicators to screen the primary formula of the product to obtain the effect of reducing the adverse feelings of the human body? The product formula is still in the exploratory stage, and there is no uniform standard method

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  • Method for detecting influence of electronic cigarette product on cell active oxygen secretion

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Embodiment Construction

[0049] The present invention will be described in detail below in conjunction with specific examples, but the present invention is not limited.

[0050] This example is aimed at testing the effects of two kinds of e-cigarette liquids commercially available abroad on the level of reactive oxygen species in human lung fibroblasts.

[0051] (1) Pretreatment of the test substance: absorb a certain amount of e-cigarette stock solution, mix and dilute it according to the volume ratio of the e-cigarette stock solution and serum-free cell culture medium at a ratio of 1:5, place it for 32 hours, and use 0.22 μm sterile Filter and sterilize to obtain the solution to be tested, and store it at -80°C until use;

[0052] (2) Preparation of single-cell suspension of human lung fibroblasts: After resuscitating human lung fibroblasts, inoculate them into cell culture flasks and place them at 37°C and 5vt% CO 2 Cultivate in an incubator, observe the confluence and morphology of the cultured c...

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Abstract

The invention discloses a method for detecting the influence of an electronic cigarette product on cell active oxygen secretion. The method includes the following steps of pretreatment of to-be-detected samples, preparation of a human lung fibroblast unicellular suspension liquid, calculation of the cell concentration of the human lung fibroblast unicellular suspension liquid, cell inoculation of the human lung fibroblast unicellular suspension liquid, to-be-detected sample grouping, to-be-detected sample detection dose setting, preparation of to-be-detected sample culture products, incubation of the to-be-detected samples, adding of a positive control, probe loading, sample measurement and active oxygen level calculation. Through the sample effective treatment, the detection dose optimized setting and the target cell correct selection, the method can effectively and accurately detect the influence of the electronic cigarette product on the cell active oxygen level.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, and in particular relates to a method for detecting the influence of electronic cigarette products on the secretion of reactive oxygen species in cells. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. E-cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. During the use of electronic cigarette products, the liquid composed of nicotine and flavoring substances is converted into a mixture similar to cigarette smoke by electric heating or electronic atomization,...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/64
CPCG01N21/6486G01N33/5008
Inventor 管莹夭建华李雪梅熊国行米其利高茜朱洲海
Owner CHINA TOBACCO YUNNAN IND
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