Preparation method of white and polysaccharide

A technology of polysaccharides and decoction pieces, applied in the field of medicine, can solve the problems of high impurities, low extraction rate of white and polysaccharides, poor color, etc., and achieve the effects of high content, clarity and good water solubility

Inactive Publication Date: 2019-04-19
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of bletilla polysaccharides, which solves the problems of low extraction rate, high impurities, and poor color of bletilla polysaccharides in the traditional method of decoction and alcohol precipitation, which cannot meet the needs of modern medical fields and in-depth research on new biological materials. Development Requirements Questions

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  • Preparation method of white and polysaccharide

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preparation example Construction

[0021] The preparation method of the whitening and polysaccharide of the present invention specifically comprises the following steps:

[0022] Step 1: Add 8 to 15 times the amount of water to the Baiji decoction pieces, soak for 60 to 90 minutes, heat soak and extract at 80 to 100°C for 60 to 120 minutes, extract 3 times, combine the 3 extracts, and extract in 4 Refrigerate at ℃, let it stand for 48 hours, separate the supernatant, discard the precipitate, and obtain the supernatant;

[0023] Step 2: Weigh the AB-8 macroporous adsorbent resin with the same quality as Baiji decoction pieces, add it to the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorbent resin in a suspended state, and keep stirring state for 2 hours, then filter and separate the AB-8 macroporous adsorption resin to obtain decolorized white and extract, and set aside;

[0024] Step 3: Concentrate the decolorized bletilla extract obtained in step 2 in a vacuum a...

Embodiment 1

[0031] Step 1: Weigh 10 parts by mass of Baiji decoction pieces, add 8 times the amount of water, soak for 60 minutes, extract by hot dipping at 100°C for 60 minutes, extract 3 times, combine the three extracts, refrigerate at 4°C, Stand still for 48 hours, separate the supernatant, discard the precipitate, and obtain the supernatant;

[0032] Step 2: Weigh the AB-8 macroporous adsorbent resin with the same quality as Baiji decoction pieces, add it to the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorbent resin in a suspended state, and keep stirring state for 2 hours, then filter and separate the AB-8 macroporous adsorption resin to obtain decolorized white and extract, and set aside;

[0033] Step 3: Concentrate the decolorized bletilla extract obtained in step 2 in a vacuum at 70°C to obtain a clear paste, put it at room temperature, add 95% ethanol to make the alcohol content reach 85%, refrigerate at 4°C, and stand for 24 ho...

Embodiment 2

[0036] Step 1: Weigh 20 parts by mass of Baiji decoction pieces, add 10 times the amount of water, soak for 80 minutes, extract by hot dipping at 90°C for 90 minutes, extract 3 times, combine the three extracts, refrigerate at 4°C, Stand still for 48 hours, separate the supernatant, discard the precipitate, and obtain the supernatant;

[0037] Step 2: Weigh the AB-8 macroporous adsorbent resin with the same quality as Baiji decoction pieces, add it to the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorbent resin in a suspended state, and keep stirring state for 2 hours, then filter and separate the AB-8 macroporous adsorption resin to obtain decolorized white and extract, and set aside;

[0038] Step 3: Concentrate the decolorized bletilla extract obtained in step 2 in a vacuum at 85°C to obtain a clear paste, put it at room temperature, add 95% ethanol to make the alcohol content reach 75%, refrigerate at 4°C, and stand for 24 ho...

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Abstract

The invention discloses a preparation method for rhizoma bletillae polysaccharide. The preparation method comprises the following steps: first, weighing rhizoma bletillae decoction pieces, performing hot-dipping extracting after water is added for soaking, refrigerating, filtering, and performing vacuum concentrating after the extracting liquid is de-coloured by using AB-8 resin; then performing alcohol precipitation, refrigerating, filtering, removing ethyl alcohol by nitrogen flow, and freezing and drying to obtain white and dry rhizoma bletillae polysaccharide, wherein the content of the rhizoma bletillae polysaccharide is about 85 percent, and the water solubility is good. According to the preparation method, the rhizoma bletillae polysaccharide is extracted by adopting a hot water dipping method; rhizoma bletillae polysaccharide degradation and high impurity content caused by overheating when the rhizoma bletillae polysaccharide is extracted according to a water decocting method are avoided; the problem that the requirements of deep research and development of modern medical treatment fields and novel biological materials cannot be met because of low rhizoma bletillae polysaccharide extracting efficiency, high impurity, poor colour and lustre in the conventional water decocting and alcohol precipitating method is solved; the problem that the rhizoma bletillae polysaccharide is in short supply at present is also solved.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method of white and polysaccharides. Background technique [0002] Bletilla striata polysaccharide (BSP) is a water-soluble polymer viscous polysaccharide extracted from the traditional Chinese medicine bletilla striata, also known as bletilla striata glucomannan, composed of D-glucose and D-mannose according to approximately The ratio of 1:4 is composed of β-glycosidic bonds to form a spatial helical structure; its average molecular weight is 65,000-150,000 kDa, and it can form a viscous gel when dissolved in water, which has good bioadhesion and is safe for the human body. It is extremely safe, and the toxicological safety evaluation shows that Baiji polysaccharide is safe, non-irritating and non-allergic. Therefore, it has a wide range of uses: ① It can be used as a food ingredient or additive, and it is an excellent natural food thickener. ②Baiji ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 秦蓓杨宽
Owner XIAN MEDICAL UNIV
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