Polysaccharide-peptide and liposome thereof

A Yunzhi glycopeptide and liposome technology, applied in the directions of liposome delivery, peptide/protein components, non-central analgesics, etc., can solve the problem of unsafe increase in production, poor water solubility of polysaccharides, and low biological activity of polysaccharides, etc. problem, to achieve excellent encapsulation rate and burst release rate, good water solubility

Active Publication Date: 2015-07-01
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional polysaccharide production process has high energy consumption, dark color of polysaccharide due to heat concentration and Maillard reaction caused by high temperature drying, poor water solubility of polysaccharide, low biological activity of polysaccharide, or consumes a large amount of alcohol to increase cost and increase production safety. Sexuality, and defects such as low polysaccharide content

Method used

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  • Polysaccharide-peptide and liposome thereof
  • Polysaccharide-peptide and liposome thereof
  • Polysaccharide-peptide and liposome thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Purified versicolor glycopeptide bulk drug

[0036]1. Source of Yunzhi fermentation broth: Trametes versicolor (Yunzhi), Trametes, preservation number CGMCC 5.161 provided by China General Microorganism Culture Collection and Management Center, the strain was transferred to slant medium for conventional culture at 26-28°C for 7 days, Get the activated Yunzhi bacterial strain; inoculate the activated Yunzhi bacterial strain on the seed medium, wherein the seed medium consists of 6% glucose, 4% yeast powder, 5% peptone, 0.2% potassium dihydrogen phosphate, and 0.02% magnesium sulfate , defoaming agent 0.1%, prepared with water, pH value is natural, 26 ~ 28 ℃ shaking culture for 3 days to obtain the first-class seed liquid of Versicolor versicolor; the first-class seed liquid is inserted into the second-level seed medium, and the second-level seed medium consists of 4% glucose, 4% yeast powder, 4% peptone, 0.2% potassium dihydrogen phosphate, 0.02% magnesium sul...

Embodiment 2

[0039] Example 2: Purified versicolor glycopeptide bulk drug

[0040] After the Yunzhi mycelium prepared according to the method of Example 1 was pulverized, 10 times the amount of purified water was added, and the water extract of Yunzhi was prepared by incubating at 95-100°C for 5 hours; the water extract of Yunzhi was micro-filtered, The membrane material used in the filtration is a ceramic membrane with a molecular weight cut-off of 400,000 Daltons. The working temperature of the microfiltration membrane is 45°C, and the working pressure: the inlet pressure is 4.0bar, and the outlet pressure is 3.0bar; the microfiltrate is taken according to 2: Add chloroform: n-butanol (volume ratio 4:1) mixture solution at a ratio of 1, shake vigorously to remove free protein, centrifuge to take the upper layer, repeat 10 times; remove the organic solvent under reduced pressure at 40°C.

[0041] For the deproteinized Yunzhi water extract, the simulated moving bed separation system is use...

Embodiment 3

[0042] Example 3: Purified versicolor glycopeptide bulk drug

[0043] Take the Yunzhi mycelia prepared according to the method of Example 1, add 10 times the amount of purified water, and keep warm at 95-100°C for 4 hours to prepare the Yunzhi water extract; the Yunzhi water extract is subjected to microfiltration, and the microfiltration The membrane material used is a ceramic membrane with a molecular weight cut-off of 450,000 Daltons. The working temperature of the microfiltration membrane is 40°C, and the working pressure: the inlet pressure is 4.0bar, and the outlet pressure is 3.0bar;

[0044] Take the microfiltrate, add chloroform:n-butanol (volume ratio 4:1) mixed solution according to the ratio of 2:1, shake vigorously to remove free protein, centrifuge to take the upper layer, repeat 10 times; remove the organic solvent under reduced pressure at 40°C .

[0045] For the deproteinized Yunzhi water extract, the simulated moving bed separation system StarSep-30025 was u...

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Abstract

The invention discloses polysaccharide-peptide and liposome of polysaccharide-peptide. Polysaccharide-peptide is prepared by the steps of extracting with water, performing micro-filtration, shaking with chloroform-n-butyl alcohol to remove the free proteins, separating, purifying, precipitating with an alcohol, and drying. The liposome of polysaccharide-peptide is prepared by using polysaccharide-peptide as the raw material, and phospholipid and cholesterol as the membrane material. The polysaccharide-peptide prepared by the invention has a polysaccharide krestin content of over 80%, is light in color, has a good water-solubility, and retains the biological activity of polysaccharide-peptide well; the liposome of polysaccharide-peptide can promote the absorption and utilization of polysaccharide-peptide in the body well and prolong the effective blood drug concentration of the drug in the body through the sustained-release effect of the liposome.

Description

technical field [0001] The invention belongs to the technical field of extraction and application of active ingredients of natural medicines, relates to a versicolor glycopeptide and its liposome, and also relates to the extraction and purification method of the versicolor glycopeptide and the preparation method of the versicolor glycopeptide liposome . Background technique [0002] Yunzhi polysaccharide (PSK) obtained from Yunzhi submerged fermentation is an anti-malignant drug (trade name: Krestin) developed by a Japanese company, which is used to treat hepatitis B, migratory hepatitis, chronic active hepatitis, etc. It is suitable for the prevention and treatment of liver cancer. Another Yunzhi glycopeptide (PSP) obtained from Yunzhi submerged fermentation can inhibit the proliferation of various cancer cells such as human lung cancer, gastric cancer, leukemia, lymphoid cancer cells and ascites cancer; it can also significantly improve the diet of tumor-bearing patients ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K9/127A61K38/00A61P35/00A61P29/00A61P37/04
Inventor 马猛华徐国华岳宗翠王英燕田智斌赵伏梅秦建丽刘杏忠董彩虹
Owner JIANGSU SHENHUA PHARMA
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