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A method for screening and enrichment culture of ammonia oxidizing bacteria with ammonia nitrogen flow addition-intermittent operation

A technology of ammonia oxidizing bacteria and enrichment culture, which is applied in the field of ammonia oxidizing bacteria flora screening and enrichment culture, can solve the problems of slow nitrification reaction rate and slow down nitrification rate, and achieve the effect of inhibiting NOB and increasing the concentration of FNA

Active Publication Date: 2020-06-16
BEIJING UNIV OF TECH
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Problems solved by technology

However, the nitrification reaction rate of the existing technology is relatively slow, and the activity of nitrifying bacteria at low temperature in winter causes the nitrification rate to slow down significantly, and the influent C / N of the sewage treatment plant is low, and an additional carbon source is required in the denitrification stage. Upgrading has extremely important economic value

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  • A method for screening and enrichment culture of ammonia oxidizing bacteria with ammonia nitrogen flow addition-intermittent operation
  • A method for screening and enrichment culture of ammonia oxidizing bacteria with ammonia nitrogen flow addition-intermittent operation
  • A method for screening and enrichment culture of ammonia oxidizing bacteria with ammonia nitrogen flow addition-intermittent operation

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Embodiment 1

[0064] 1. Determination of ammonia nitrogen oxidation rate of inoculated sludge

[0065] Take sewage treatment plant A 2 / O process reflux sludge is inoculated sludge, and the fully automatic bacterial fermentation tank is used as the test device, with an effective volume of 3.5L, such as figure 1 shown. The sludge concentration (MLSS) of the inoculum sludge was 3970mg / L. Temperature 25±1℃, pH8.1~8.4, stirring speed 120rpm, Na 2 CO 3 The stock solution concentration is 200g / L. One-time dosing of ammonia nitrogen (NH 4 Cl) Intermittent operation to measure the ammonia nitrogen oxidation rate of inoculated sludge, DO0.8~1.1mg / L, initial NH 4 + -N concentration is [NH 4 + -N] 开始 54.43mg / L, reaction time t 1 For 2h, the reaction ends with NH 4 + -N concentration is [NH 4 + -N] 结束 is 24.58mg / L, and the ammonia nitrogen oxidation rate AOR of inoculated sludge is calculated according to formula (1) to be 14.93mg / (L·h).

[0066] 2. Ammonia-nitrogen fed-batch operation...

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Abstract

The invention relates to an ammonia nitrogen feeding-periodic running ammonia-oxidizing bacterium colony screening and enrichment culture method, and belongs to the technical field of water treatment. The screening and enrichment culture of an ammonia-oxidizing bacterium colony are realized by taking activated sludge containing ammonia-oxidizing bacteria as inoculated sludge by means of a bacterium fermentation tank through an ammonia nitrogen feeding-periodic running method. Free ammonia and free nitrous acid are used to inhibit nitrite-oxidizing bacteria, and high dissolved oxygen contributes to improving the ammonia nitrogen oxidation rate. The screening and enrichment culture of the ammonia-oxidizing bacterium colony can be realized within short time according to the method. The obtained ammonia-oxidizing bacterium colony has a numerical advantage in the total bacteria, thereby achieving high ammonia nitrogen oxidation rate and high nitrite accumulation rate.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for screening and enriching culture of ammonia-oxidizing bacterial flora with ammonia nitrogen feeding-intermittent operation. Background technique [0002] Saving aeration energy consumption and organic carbon sources as much as possible in the process of biological denitrification is an effective way to achieve sustainable development of sewage biological treatment. The traditional biological nitrogen removal process is autotrophic nitrification and heterotrophic denitrification. (1) NH is converted by ammonia-oxidizing bacteria (AOB) 4 + Oxidation to NO 2 - ; (2) by nitrite-oxidizing bacteria (Nitrite-oxidizing bacteria, NOB) NO 2 - Oxidation to NO 3 - ; (3) denitrifying bacteria will NO 3 - Revert to N 2 . The first two steps are called full-scale nitrification, and the first step is called short-cut nitrification. In the sewage denitrification...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01
CPCC02F3/34C12N1/02C12N1/20
Inventor 杨宏姚仁达
Owner BEIJING UNIV OF TECH
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