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Preparation method of an antibiotic self-powered aptamer sensor

An aptamer sensor and antibiotic technology, which is applied in the fields of instruments, scientific instruments, electrochemical variables of materials, etc., can solve the problems of cumbersome operation, high analysis cost, lengthy steps, etc., and achieve simple detection equipment, portable integration, high selective effect

Inactive Publication Date: 2019-04-30
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many antibiotic drug residue tests, the accuracy is poor and the sensitivity is not enough; or the steps are lengthy and the operation is cumbersome; or rely on large instruments, the analysis cost is high, and it is difficult to meet the growing demand for accurate, simple, fast and highly sensitive detection

Method used

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  • Preparation method of an antibiotic self-powered aptamer sensor
  • Preparation method of an antibiotic self-powered aptamer sensor
  • Preparation method of an antibiotic self-powered aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Enzyme biofuel cell-based self-powered antibiotic biosensor for the detection of ampicillin (AMP)

[0032] (1) SiO 2 Preparation: Add 4.5 mL of tetraoxysilane (TEOS) to a mixed solution of 9 mL of ammonia water (28%), 16.26 mL of ethanol and 24.75 mL of water, stir at room temperature for 3 h, centrifuge and wash with excess TEOS and ammonia water, Dry at 60 °C. Subsequently, 0.4 mL of aminopropyltriethoxysilane (APTES) was added, stirred at room temperature for 6 h, washed and dried;

[0033] (2) SiO 2 Preparation of @AuNPs: 500 µL of AuNPs at a concentration of 10 nM was added to 100 µL of 1 mg mL -1 Aminated SiO 2 solution, incubate overnight, centrifuge to remove excess AuNPs, and constant volume to obtain 1 mg mL -1 SiO 2 @AuNPs;

[0034] (3) SiO 2 Assembly of @AuNPs-csDNA: 1 μM dithiothreitol (DTT)-activated aptamer partially complementary DNA strand (csDNA) was mixed with 1 mg mL -1 SiO 2 @AuNPs were incubated at room temperature for 2 h, the...

Embodiment example 2

[0041] Implementation case 2: Enzyme biofuel cell-based self-powered antibiotic biosensor for detection of tetracycline (TC)

[0042] Aptamer to DNA sequence TC: 5'-NH 2 -(CH 2 ) 6 -TTT TCG TAC GGA ATT CGC TAG CCC CCCGGC AGG CCA CGG CTT GGG TTG GTC CCA CTG CGC GTG GAT CCG AGC TCC ACG TG-3'

[0043] ssDNA: 5'-SH-(CH 2 ) 6 -TTT TTT TTT CAC GTG GAG CTC GGA TCC ACG CGC-3'

[0044] All the other steps are with embodiment 1.

[0045] 5'-NH 2 -(CH 2 ) 6 -TTT TGC GGG CGG TTG TAT AGC GG-3'

[0046] 5'-SH-(CH 2 ) 6 -TTT TTT TTT CCG CTA TAC AAC CGC C-3'

[0047] 5'-NH 2 -(CH 2 ) 6 -TTT TCG TAC GGA ATT CGC TAG CCC CCC GGC AGG CCA CGG CTTGGG TTG GTC CCA CTG CGC GTG GAT CCG AGC TCC ACG TG-3'

[0048] 5'-SH-(CH 2 ) 6 -TTT TTT TTT CAC GTG GAG CTC GGA TCC ACG CGC-3'

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Abstract

The invention relates to a preparation method of an antibiotics self-powered aptamer sensor. The preparation method is used for detecting antibiotics according to the change of output property of an EBFC (enzymatic biofuel cell). The design of the self-powered sensing is mainly focused on an anode of the EBFC, a DNA (deoxyribonucleic acid) conjugate is modified to the surface of the anode, the steric hinerance influences the proton transport of glucose of the anode fuel, and the open-loop voltage is lower. When the target antibiotics exists, the aptamer modified to the surface of the anode is used for identifying the antibiotics medicine, and the DNA conjugate is disengaged, so that the anode can effectively catalyze the oxidizing of the glucose, the open-loop voltage of the EBFC is increased, the change value of open-loop voltage forms a proportional relationship with the concentration of the antibiotics, so that the antibiotics medicine is detected. The preparation method has the advantages that the additional power supply equipment is not needed in the sensor detection process, the cost is low, the anti-interference ability is strong, and the sensor has high selectivity by using the aptamer identifying function. The antibiotics self-powered aptamer sensor can be used for realizing the simple, quick, sensitive and efficient detection of the antibiotics medicine.

Description

technical field [0001] The invention relates to a preparation method of an antibiotic self-powered aptamer sensor based on an enzyme biofuel cell. The presence of antibiotics affects the performance of enzyme biofuel cells. Through the change of open circuit voltage of enzyme biofuel cells, the detection of target antibiotic drugs is simple, fast, sensitive and efficient. Background technique [0002] Enzyme biofuel cell (EBFC) is a special fuel cell that utilizes renewable fuels to provide sustainable energy under the catalysis of biological enzymes. EBFC-based self-powered biosensors are a class of sensors whose analyte concentration is directly proportional to the battery output signal. Compared with traditional sensors, self-powered biosensors do not need to apply additional power during the detection process, and their specific advantages are mainly as follows: (1) The equipment is simple. The detection process is different from the traditional electrochemical detecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3275
Inventor 盖盼盼侯婷李峰
Owner QINGDAO AGRI UNIV
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