30 results about "Proton transport" patented technology

The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.

The invention discloses a preparation method of a polypropylene cation exchange membrane, which effectively achieves the purpose of enhancing the chemical stability of the membrane, and comprises the following steps: step S1, carrying out plasma treatment on a polypropylene base material, and synchronously grafting a sulfonic acid group-SO3H and a quaternary ammonium group-N + R3 under photochemical reaction conditions; s2, mixing and dispersing the surface-functionalized polypropylene master batch and a sulfonated two-dimensional carbon material; s3, mixing the complex with an aprotic polar solvent, and forming a porous skeleton through thermally induced phase separation; s4, compounding the porous framework with cation exchange resin. The method is ingenious in conception and simple and convenient to operate, the purposes of effectively constructing a continuous proton transmission channel and increasing the ionic conductivity are achieved, a nano-channel network with low tortuosity is formed, and the purposes of proper membrane strength and flux attenuation rate are achieved.

This membrane electrode assembly comprises: a proton conductor (10); and a catalyst layer (20) that is provided so as to sandwich the proton conductor and that is joined to the proton conductor. Provided to the proton conductor is a recessed-and-protruding section (12) where portions of the catalyst layer penetrate a catalyst bonding surface (11) to which the catalyst layer is bonded.　In the catalyst layer, a region where the proton transport resistance increases proportionally with separation from the catalyst bonding surface is defined as a high-resistance region (HA). Furthermore, in the catalyst layer, a region where the proton transport resistance is smaller than that of the high-resistance region and the degree of increase in the proton transport resistance accompanying separation from the catalyst bonding surface is smaller than that of the high-resistance region is defined as a low-resistance region (LA). As a result, the dimensions of at least the recessed-and-protruding section are set such that the proportion of the entire catalyst layer occupied by the low-resistance region is larger than when the catalyst bonding surface is a flat surface having no recesses or protrusions.

The present invention relates to a squid-derived conductive protein and a method for manufacturing same. Having a sequence partially similar to genetic information of a reflectin protein possessing a proton transport mechanism, the squid-derived conductive recombinant protein exhibits electrochemical properties corresponding to an in vivo electrical signal transmission mechanism, can be mass-produced from a transformant, can be used as a new material based on electrochemical properties, and can be formulated into various chemical formulations, thereby finding applications in a flexible electrode, an electrolyte, an active material, and the like.

The invention relates to fuel cells and electrolyzer electrodes comprising non-ionomer binders. An electrode of an electrochemical device includes a carbon-based support structure, an ion conducting material dispersed on and within the support structure, a catalyst dispersed on and within the support structure, and a non-ionomer hydrocarbon-based binder dispersed on the support structure. The ion conducting material enables proton transport across the electrode, the catalyst promotes a chemical reaction of the fuel received at the electrode, and the binder holds the electrode. The electrode may be included in an electrochemical device, such as a fuel cell.

The present disclosure provides a discretization modeling method for electro-osmotic drag effect of water conservation in a fuel cell, comprising: establishing a conservation equation of membrane water in the fuel cell, performing a discretization for a complete electro-osmotic drag effect, obtaining a discretization simulation model of the complete electro-osmotic drag effect based on results of the discretization, solving the conservation equation of membrane water to establish a discretization simulation model of electro-osmotic drag effect of water conservation in the fuel cell. The discretization modeling method for the electro-osmotic drag effect of water conservation in a fuel cell in the present disclosure can perform a discretization and a numerical calculation for a complete electro-osmotic drag effect, the discretization comprising a water conservation portion caused by a membrane water content gradient and a water conservation portion caused by a proton transport flux gradient.

The present application relates to a kind of Ce-MOF doped Nafion composite proton exchange membrane and its preparation method and purposes, the Ce-MOF doped Nafion composite proton exchange membrane includes Nafion composite proton exchange membrane and Ce-MOF;Metal site in the Ce-MOF is Ce;The amino group is contained in the Ce-MOF.By introducing Ce element, using Ce as radical quencher, effectively eliminate free radicals, reversible redox pair Ce 3+ And Ce 4+ , inhibit polymer chain chemical degradation, help to improve the durability of proton exchange membrane, improve the oxidation resistance of proton exchange membrane.Simultaneously through MOF skeleton anchoring Ce ion position, effectively solve the problem of Ce loss after long time work, while introducing amino and sulfonic acid group on Nafion chain form proton transport channel, ensure the proton conductivity of membrane.

The application discloses a kind of double-gate graphene proton transport device in-situ test system and test method under strong magnetic field, the system is double-layer structure, comprising: inner rod, for placing double-gate graphene proton transport device, the inner rod is integrated with temperature control system, for realizing 100mK to 300K variable temperature test environment;Outer rod, it is set in the outer portion of the inner rod, for independently controlling the vacuum or specific atmosphere environment where the device is located.The application also provides a test method, the assembled system is placed into magnetic field environment, scanning is carried out in predetermined magnetic field intensity range, and temperature is controlled to change between 100mK to 300K;Through the first electrical measurement unit and second electrical measurement unit, the curves of proton current and electron current change with magnetic field, temperature and gate voltage are recorded simultaneously.By unique device structure design and system integration scheme, it provides a powerful tool for studying atomic scale proton transport behavior under extreme multi-physical field coupling environment.

The present application relates to a kind of proton exchange membrane and its preparation method, including first exchange layer, intermediate layer and second exchange layer, intermediate layer includes support layer, ion exchange material filled in support layer and water hole, equivalent weight Ew of ion exchange material is not higher than 1600, the thickness H of proton exchange membrane in dry state 干 Not higher than 17 μm, the compactness I of proton exchange membrane is 0.15-0.65, the mass m of support layer per unit area in dry state P 2-10 g;The thickness h of support layer in dry state p 2-8 μm.The existence of water hole reduces water permeation resistance, reduces the difficulty of proton membrane forming proton transport channel, reduces the possibility of water flooding under high humidity condition, the possibility of proton transport channel circuit breaking under high temperature and low humidity condition, ensures high proton conductivity;In addition, the existence of water hole provides ion exchange material swelling deformation space, thereby improving the dimensional stability of proton membrane.

This invention discloses an aqueous zinc-ion battery electrolyte containing aminopyrine as an additive. The electrolyte comprises a water-soluble zinc salt, deionized water, and an additive, aminopyrine, at a concentration of 1-20 mmol / L. The aminopyrine of this invention reacts with H3O through its sulfonic acid group. + The formation of a strong hydrogen bond network significantly suppresses proton transport, which synergistically achieves triple suppression of hydrogen evolution reaction, corrosion and dendrite growth, ultimately guiding uniform zinc deposition and improving battery cycle stability.

This invention discloses a method for preparing a non-fluorinated composite proton exchange membrane. The method uses sulfonated polyether ether ketone (PEEK) as the matrix and incorporates two specific sulfonated covalent organic framework materials. The addition of the sulfonated covalent organic framework forms a continuous hydrogen bond network and highly efficient proton transport channels within the membrane, significantly improving proton conductivity. The composite membrane achieves a proton conductivity of up to 0.41 S / cm at 80°C, approximately three times higher than the unmodified matrix. Simultaneously, the rigid structure of the covalent organic framework effectively enhances the membrane's mechanical strength, increasing its tensile strength to 75 MPa, achieving a synergistic enhancement of proton conductivity and mechanical properties. The non-fluorinated composite proton exchange membrane prepared by this invention exhibits a stable composite membrane structure and excellent overall performance, making it suitable for applications such as proton exchange membrane water electrolysis for hydrogen production.

The present invention provides an electrode catalyst layer, a membrane electrode assembly, and a polymer electrolyte fuel cell that can enhance power generation performance over a wide load range by suppressing catalyst poisoning and improving proton transport using ionomers. [Solution] The electrode catalyst layer 12C of the air electrode includes a catalyst support 20 which is a porous carbon support 21 supporting a catalyst substance 23, and a polymer electrolyte 24, and the ECSA ratio, which is the ratio of ECSA at a relative humidity of 30% to ECSA at a relative humidity of 100%, is 0.55 or more and 0.65 or less. The catalyst support 20 includes a first catalyst support 20a which has an ECSA ratio of 0.5 or less when used alone as the catalyst support 20 included in the electrode catalyst layer 12C, and a second catalyst support 20b which has an ECSA ratio of 0.7 or more when used alone as the catalyst support 20 included in the electrode catalyst layer 12C.

The disclosure of the application provides a membrane design encompassing three main considerations; (1) employing 2D nanosheets as proton-permeable building blocks to facilitate through-membrane proton transport; (2) assembling 2D nanosheets to form 2D channels, capable of confining the proton conductor to thereby prevent loss of the proton conductor, or to prevent physical or chemical degradation of the proton conductor under the conditions in which the membrane may be exposed in its application(s); and (3) incorporating proton conductors to occupy the intersheet spaces forming the 2D channels, and / or incorporating proton conductors to form at least part of the inner surfaces of the 2D channels, enabling intersheet proton transport while blocking hydrogen gas molecules, thereby providing membranes with ultrafast proton conducting pathways due to synergistic proton transport, with high retention of the proton conductor achieved through nanoconfinement in 2D channels.

This invention discloses a polyaromatic hydrocarbon for preparing proton exchange membranes, its preparation method, and its application, belonging to the field of proton exchange membrane material preparation technology. This invention solves problems such as low molecular weight, uneven sulfonation, chain breakage in sulfonated polymers, poor dimensional stability, and poor mechanical properties in proton exchange membrane materials. Using aromatic monomers and 3,5-difluorotrifluoroacetophenone as raw materials, this invention employs a superacid-catalyzed Friedel-Crafts hydroxyalkylation reaction to prepare fluorinated aromatic polymers. Then, sulfonic acid groups are introduced onto the fluorinated aromatic polymers via nucleophilic substitution followed by sulfonation, preparing monosulfonated and disulfonated fluorinated sulfonic acid polymers. Proton exchange membranes are then prepared using these polymers as raw materials. The significant polarity difference between the hydrophobic fluorinated benzene ring side groups and the hydrophilic disulfonic acid groups in this invention is more conducive to forming a microphase separation structure and constructing continuous proton transport channels. Simultaneously, the hydrophobic fluorinated segments effectively suppress membrane swelling and improve its dimensional stability.

This invention provides a method for preparing an anode catalyst layer for PEM water electrolysis. By doping additives into the catalyst layer, not only are proton transport channels between catalysts constructed, reducing the proton transport impedance between catalysts and improving the electrochemical reaction rate, but the additives also disperse the catalyst, exposing more active sites, increasing catalyst utilization, and improving the performance of PEM water electrolysis. Furthermore, by separating the additive slurry and the anode catalyst slurry and using dual-channel ultrasonic spraying to control the feeding of each slurry, the layered composition of the catalyst layer can be efficiently and quickly adjusted to prepare a catalyst layer with the desired structure and composition distribution, eliminating the need for multiple slurry preparations and sprayings. This process offers high flexibility and a short cycle time. By preparing a catalyst layer with gradients in catalyst loading and sulfonated silica content through layering, not only is catalyst utilization improved, but also efficient electron and proton transport channels are constructed, improving the water electrolysis rate.

This invention provides a ceramic proton exchange membrane for microbial fuel cells and its preparation method, comprising the following steps: mixing clay, an inorganic modifier, and an organic modifier to obtain a solid powder; the inorganic modifier is alumina and silicon dioxide, and the organic modifier is selected from one or more of wheat flour, biochar powder, straw powder, and bamboo powder; the solid powder is ball-milled, granulated, pressed into sheets, and calcined to obtain the ceramic proton exchange membrane. This invention addresses the problems of poor mass transfer performance and low power generation performance of traditional pure clay ceramic membranes. Using clay as the matrix material, and through specific inorganic and organic modifiers, the pore structure of the material is adjusted. A novel ceramic proton exchange membrane is then prepared through ball milling, granulation, pressing, and calcination. This membrane has low manufacturing cost, good proton transport efficiency, and extremely low internal resistance. When applied to microbial fuel cells, it can achieve higher output voltage and more stable power generation performance.

The invention provides a proton track and dose simulation method based on prevention capability and random scattering. The method comprises the following steps: S1, reading proton prevention capability, continuous moderated approximate range and detour factor data of a target material; s2, generating a self-adaptive scattering step length suitable for the current energy by using the basic radiation parameter table; s3, optimizing empirical parameters in the scattering angle correction factor by taking the detour factor as a criterion; s4, calculating a scattering angle standard deviation, and generating a single scattering angle conforming to Gaussian distribution; s5, calculating a single scattering track and energy loss of the protons; s6, iteratively simulating single scattering and energy loss calculation until the kinetic energy of the proton is zero or the proton passes through the target material; and S7, outputting a proton complete track and a dose distribution curve. According to the method, the proton blocking threshold curve and the scattering theory are combined, the correctness of the particle scattering statistical behavior is ensured by taking the detour factor as the criterion, and rapid simulation and accurate calculation of the proton transport track and the radiation dose are realized.

This invention discloses a method and system for real-time pH control and stabilization in online column separation, belonging to the field of ion exchange chromatography separation technology. The method includes: applying a proton perturbation signal to the chromatographic column and detecting its elution response signal to obtain a parameter spectrum characterizing the dynamic buffer capacity of the chromatographic column; inputting the parameter spectrum and the target pH gradient into a physical transport model based on convection, diffusion, and adsorption coupling equations; the physical transport model outputs a pre-distorted pH gradient program to compensate for the dynamic buffer capacity characteristics; and based on the fluid ratio requirements defined by the pre-distorted pH gradient program and varying over time. This allows for the acquisition of dynamic buffer capacity characteristics through proton perturbation testing and the construction of a pre-distorted pH gradient program. Combined with closed-loop iterative optimization, this effectively counteracts the interference of the chromatographic column's dynamic buffer capacity on proton transport, ensuring that the pH curve of the post-column elution accurately matches the target linear gradient and eliminating pH nonlinear distortion and plateau problems.

This invention discloses a method for preparing fuel cell catalyst slurry. By combining long-branched resin solutions and short-branched resin solutions with different EW values, and employing a unique dispersion method, the catalyst is uniformly dispersed in the slurry, improving catalyst utilization efficiency. This results in better proton transport under low electrical density conditions and maintains hydrophobic properties under high electrical density conditions, reducing the risk of flooding. The catalyst slurry prepared by this invention, after being fabricated into a membrane electrode assembly (MEA), can well adapt to the usage requirements under different humidity conditions, solving the problems of low-humidity start-up and high-humidity flooding in fuel cells, and ensuring the performance and service life of the MEA.

The application provides a proton track and dose simulation method based on stopping power and random scattering, comprising the following steps: S1. reading proton stopping power, continuous slowing-down approximation range and data of the target material; S2. using a basic radiation parameter table to generate an adaptive scattering step length suitable for the current energy; S3. taking the bypass factor as a criterion to optimize the empirical parameters in the scattering angle correction factor; S4. calculating the standard deviation of the scattering angle to generate a single scattering angle conforming to the Gaussian distribution; S5. calculating the single scattering track and energy loss of the proton; S6. iteratively simulating the single scattering and energy loss calculation until the kinetic energy of the proton is zero or the proton passes through the target material; and S7. outputting the complete track of the proton and the dose distribution curve. The application combines the proton stopping power curve and the scattering theory, takes the bypass factor as a criterion to ensure the correctness of the statistical behavior of particle scattering, and realizes the rapid simulation and accurate calculation of the proton transport track and radiation dose.

This invention discloses a titanium dioxide-coated oxygen-defect-containing molybdenum trioxide material, its preparation method, and its applications. The titanium dioxide-coated oxygen-defect-containing molybdenum trioxide material (MoO) 3‑x The preparation method of TiO2 is as follows: First, TiO2 is coated onto the surface of MoO3 using tetrabutyl titanate as the titanium source. Then, low-carbon fatty alcohols such as methanol and ethanol are used as reducing agents to introduce oxygen vacancies. The TiO2 coating layer avoids the formation of oxygen vacancies on the surface of MoO3. 3‑x Contact with the electrolyte inhibits dissolution and confines MoO₂. 3‑x This allows it to maintain structural stability during repeated proton insertion / extraction processes. The introduction of oxygen vacancies can increase the interlayer spacing of MoO3 and improve the MoO2 content. 3‑x The conductivity of TiO2 promotes proton transport dynamics. Therefore, MoO 3‑x @TiO2, as a proton storage electrode material, combines high capacity, high rate capability, and good cycling stability.

The invention discloses a preparation method of a proton type ionic liquid polymer (at) MOFs (at) PVDF composite proton exchange membrane, and belongs to the technical field of fuel cells. According to the proton exchange mixed matrix membrane, a proton type ionic liquid polymer monomer containing a sulfonic acid group is subjected to in-situ polymerization in a pore channel of a metal-organic framework with sulfonic acid functionalization, and a PVHim-HSO4 (at) MIL-101-SO3H composite material is prepared. The composite material and polyvinylidene fluoride form a novel composite proton exchange membrane. The composite proton exchange membrane disclosed by the invention not only keeps relatively high proton conductivity, but also shows excellent mechanical properties, and effectively solves the problems of low proton transmission efficiency and insufficient mechanical strength of a traditional proton exchange membrane. The proton exchange membrane is applied to a fuel cell, a more efficient and stable energy conversion process is expected to be realized, and a new technical support is provided for research and development of a high-performance fuel cell.

The invention discloses a preparation method and application of an electrochemically modified Ti3C2 MXene film, and belongs to the field of nano materials and supercapacitors. According to the preparation method disclosed by the invention, the single-layer Ti3C2 MXene is prepared by etching the Ti3AlC2 precursor, and the improvement of the yield and the quality of the single-layer MXene by prolonging the etching time (10-40 days) is innovatively proposed, so that the self-supporting Ti3C2 MXene film with strong toughness is prepared. The thin film is used as a working electrode, a platinum wire is used as a counter electrode, an Ag / AgCl electrode is used as a reference electrode, a saturated sulfate solution is used as an electrolyte, charging and / or discharging are / is carried out on an electrochemical workstation at a certain scanning rate, and the electrochemically modified Ti3C2 MXene thin film is obtained after washing and drying. According to the electrochemical modification method, the Ti3C2 MXene can obtain an interlayer structure formed by a small amount of cations and a water column support, diffusion of electrolyte ions cannot be hindered due to existence of a large amount of ions in spontaneous intercalation, and interlayer water can accelerate transmission of protons, so that the capacitance of the MXene can be greatly improved. When the saturated sulfate solution is Cs2SO4 and the scanning rate is 5 mV / s, the capacitance of the Ti3C2 MXene film electrode is increased to 150.1 F / g from 82.8 F / g, and the amplification reaches 67.3 F / g.

The present invention provides technologies relating to modulation of voltage-gated ion channels. Particularly, the present invention provides pharmaceutical compounds for inhibiting ion transport by a voltage-gated ion channel in a subject in need thereof. In certain embodiments of the invention, the ion is a proton and the voltage-gated ion channel is a voltage gated proton channel such as Hv1. The invention further provides methods of administering a therapeutically effective amount of the pharmaceutical compounds to a subject to block the proton transport by a voltage-gated proton channel such as Hv1.

This invention discloses a conductive MOF-functionalized gel and its preparation method, applicable to electroencephalogram (EEG) signal transduction, belonging to the field of medical devices. The functionalized gel of this invention uses a conductive MOF material as a proton transport matrix, further covalently grafted onto a hydrophilic polymer to obtain a MOF-modified polymer material. The modified polymer is then used as the conductive and gel matrix to prepare the gel material. This method improves the dispersibility and stability of MOFs in the gel, enhancing the stability of gel-based electrical signal transduction. The conductive MOF-functionalized gel material of this invention can be used as a conductive gel for electrical signal transduction in EEG signal monitoring during anesthesia.

This invention relates to the field of zinc-ion battery technology, specifically to an electrolyte and a zinc-ion battery containing the electrolyte. The electrolyte provided by this invention comprises a zinc salt, water, and an additive composition. The additive composition comprises a calcium salt and an amine compound containing three or more hydroxyl groups. The calcium salt provides Ca... 2+ The calcium salt has a strong attraction to electronegative oxygen atoms in H₂O, which can lock in free water molecules in the bulk electrolyte, thereby forming a large solvation sheath, breaking the original hydrogen bond network between water molecules, and thus lowering the freezing point of the electrolyte. However, the calcium salt only acts on the bulk electrolyte and cannot alleviate the capacity loss caused by the slow ion diffusion kinetics at the positive electrode interface. More importantly, during cycling, calcium ions may embed between the MnO₂ material layers, blocking proton transport channels, leading to structural collapse and capacity decay.

The utility model discloses a multi-layer homogeneous microporous proton exchange composite membrane which comprises a composite membrane body, a substrate is arranged in the composite membrane body, a strengthening layer is arranged on the outer surface of the substrate, a proton transmission layer is arranged on the outer surface of the strengthening layer, a methanol blocking layer is arranged on the outer surface of the proton transmission layer, and the methanol blocking layer is arranged on the outer surface of the methanol blocking layer. And an optimization layer is arranged on the outer surface of the methanol blocking layer. According to the device, the substrate and the strengthening layer serve as a supporting structure of the composite membrane, the performance of the composite membrane is improved, stable supporting and effective protection of the composite membrane are achieved, the proton transmission layer, the methanol blocking layer and the optimization layer are matched, electrochemical reaction is guaranteed through the proton transmission layer, and meanwhile interference of other substances is prevented; the performance and stability of the fuel cell are improved, the methanol blocking layer prevents methanol molecules from passing through, the energy conversion efficiency and stability of the cell are improved, the optimization layer improves the performance of the membrane and adjusts interaction between layers, and then the composite membrane has high practicability.

The application discloses a kind of double wide area-high acid retention gel state mixed matrix PBI proton exchange membrane and its preparation method and application, the gel state proton membrane is by flexible zirconium-glutamic acid (L-Glu-Zr (P)) or UiO-66 series metal organic framework, polybenzimidazole (PBI), phosphoric acid and water composition, its preparation method includes: with polyphosphoric acid as solvent, under the protection of inert gas, aromatic tetraamine monomer, dicarboxylic acid phenyl monomer high-temperature polycondensation forms PBI polymer solution, adds metal organic framework particle and disperses uniformly, mixed solution is scraped on substrate and experiences from solution to gel state membrane The phase transition process;Proton exchange membrane prepared in the application all shows excellent acid retention capacity, proton transport characteristics, fuel cell performance and stability under the service window of double wide area (temperature-20~240 DEG C, humidity 0~80%RH), has very good application prospect in fuel cell field.

The invention discloses a proton exchange membrane electrolyzed water anode catalyst layer with a cross-linked ionomer network and a preparation method of the proton exchange membrane electrolyzed water anode catalyst layer, and the preparation method comprises the following steps: preparing primary slurry 1, preparing primary slurry 2, preparing final anode catalyst slurry, then placing a proton exchange membrane on a constant-temperature heating table, spraying the final anode catalyst slurry on the proton exchange membrane, and drying to obtain the proton exchange membrane electrolyzed water anode catalyst layer with the cross-linked ionomer network. And completely drying to obtain the proton exchange membrane electrolyzed water anode catalyst layer with the cross-linked ionomer network. By changing the controllable dielectric constant of the slurry, the dispersion behavior of the ionomer in the slurry is controlled, so that the component distribution of the catalyst layer is controllable; and meanwhile, through a multi-stage thickening agent adding process, a non-mutation stable transition process is formed, so that the stability of the slurry is remarkably enhanced while all dispersed phases in the slurry are always kept uniformly distributed, and an anode catalyst layer structure which can be repeatedly and uniformly prepared and is provided with an efficient proton transmission network is formed. Compared with a traditional catalyst layer structure, the anode catalyst layer with the cross-linked ionomer network prepared according to the application shows low mass transfer resistance and low activation impedance, so that a membrane electrode still keeps low voltage loss under high current density, and the water electrolysis efficiency can be effectively improved.

The invention discloses an air-cooled fuel cell catalyst layer with a high-efficiency proton transport network and a preparation method thereof, relates to an air-cooled proton exchange membrane fuel cell catalyst layer and a preparation method thereof, and aims to solve the problems of poor cell performance and low stability of the conventional air-cooled fuel cell catalyst layer at low humidity. A first catalyst layer of the air-cooled fuel cell catalyst layer is composed of a platinum catalyst with a nanoscale conductive oxide as a carrier and an ionomer; the second catalyst layer consists of a platinum-carbon catalyst taking modified mesoporous carbon as a carrier, an ionomer and a hydrophilic conductive additive; wherein the platinum-carbon catalyst is prepared by modifying mesoporous carbon through mixed acid hydrophilization and laser fluorination hydrophobic treatment and depositing platinum nanoparticles in pores in a confinement manner based on a pressure-assisted capillary effect. The low-humidity condition single cell output performance of the catalyst layer is 0.734 V at 500 mA / cm < 2 >, the air-cooled short reactor output performance is 0.729 V at 500 mA / cm < 2 >, and the catalyst layer can be applied to the field of air-cooled fuel cells.