54results about How to "Simple detection equipment" patented technology

The chip is composed of two pieces of basis materials being bonded. The mini pump without valve, the in and out flow channels as well as intercrossed the electrophoresis separation channel and the electrophoresist sample channel are setup in the adjoining plane. The one end of the electrophoresis sample channel and the in flow channels are connected each other in 'T' form. The mini pump without valve is utilized as the automatic sample change unit in electrophoresist continuous measuring multiple samples. With having been measured, the former sample is discharged to the waste tank. Meanwhile the new sample is fed to the sample introduction point. The chip can be repeatable use so as to simplify the test device, shorten the continuous measuring time for multiple samples, lower the operation requirements.

The invention discloses a method for quantitatively detecting mutant DNA by combining GQDs-based resonance light detection probes with CHA technology. The invention firstly synthesizes graphene quantum dots, then designs two kinds of hairpin probes H1 and H2 for the target object, and then prepares two kinds of probes GQDs‑H1 and GQDs‑H2 through condensation reaction of the probes and GQDs. In the PBS buffer solution reaction system, when p53 mutant DNA is added, the aggregation of GQDs-H1 and GQDs-H2 will be caused, and a significantly enhanced resonance light signal will be generated to realize the quantitative detection of p53 mutant DNA. The invention has high sensitivity and selectivity, the linear range is 1pM to 50nM, the detection limit is 0.8pM, and the target object can be quantitatively detected effectively.

The invention discloses a reagent kit and a method for rapidly, qualitatively and quantitatively detecting microcystic aeruginosa on spot, detecting by using a nucleic acid probe with a fluorescein isothiocyanate added at the 5'end. The detection method comprises the following steps: firstly fixing algae cells; dehydrating and hybridizing, and cleaning and observing the algae cells under a fluorescence microscope.The invention has the advantages of correctness, rapidness and simpleness, and has simple operation because less than 20 hours is required for the whole detection process. The most important is that detection can be performed on spot, and qualitative analysis of the algae cells can be carried out in natural water body, which can not be achieved by the prior other methods for authenticating microcystis flos-aquae, therefore, the invention has favorable application prospect on detection of the microcystis flos-aquae.

The invention belongs to the field of preparation and application of nano materials, and discloses a composite nano material capable of exciting upconversion steady persistence through near-infrared light, and preparation method and application of the composite nano material. By utilizing the composite nano material disclosed by the invention, the situation that nanoparticles are excited by near-infrared wavelength light to generate steady persistence luminescence is realized. The composite nano material is of a special composition structure which is obtained through combination of an upconversion nano material and a steady persistence nano material. According to the composite nano material disclosed by the invention, the situation that visual light emitted the upconverision nano material after near-infrared light is absorbed by the upconversion nano material is matched with absorbed light of the steady persistence nano material, i.e., the visual light emitted by the upconversion nano material is absorbed by the steady persistence nano material, so that light emitting of the steady persistence nano material is realized, the generation of steady persistence luminescence under the exciting of the near-infrared light is realized, the problems of a traditional steady persistence nano probe that the penetrating power of short-wavelength laser is weak, materials in a living organism cannot be excited, and the like are overcome, and deep optical imaging with continuous detection, high SNR (Signal to Noise Ratio) and a simple detecting device and without background exciting light, tissue autofluorescence, and a light filter is realized.