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Rapid and sensitive plasmodium falciparum detection method

A Plasmodium falciparum and detection method technology, applied in the field of in vitro diagnostic reagents, can solve problems such as complex operations, and achieve the effects of improving detection capabilities, reducing time and complexity, and quickly and accurately detecting

Inactive Publication Date: 2015-04-22
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The current gene chip detection for malaria parasite detection has been used in some researches, but the probe spotting of these gene chips mostly adopts manual mode, which is complicated to operate and increases the chance of many false positives

Method used

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  • Rapid and sensitive plasmodium falciparum detection method
  • Rapid and sensitive plasmodium falciparum detection method
  • Rapid and sensitive plasmodium falciparum detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The specificity and sensitivity of the detection of embodiment 1 Plasmodium falciparum

[0043] 1. Experimental steps

[0044] 1. Collection of blood samples

[0045] Take 1.0ml of whole blood sample from the patient at the port for testing.

[0046] 2. Preliminary Identification of Blood Samples

[0047] The colloidal gold method was used to preliminarily determine whether the patient was infected with Plasmodium falciparum.

[0048] 3. Extraction of Genomic DNA

[0049] Plasmodium falciparum DNA was extracted using a blood sample genomic DNA extraction kit (spin column type, Qiagen). Take 50uL whole blood sample, and extract DNA according to the extraction steps in the kit manual.

[0050] 4. Determination of DNA concentration

[0051] The concentration of Plasmodium DNA was quantified by real-time quantitative PCR using ABI's real-time fluorescence detector.

[0052] 5. Amplification of target genes

[0053] 5.1 Design of primers and probes

[0054] Aiming at ...

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Abstract

The invention relates to a rapid and sensitive plasmodium falciparum detection method. The detection method integrates two powerful molecular biology techniques of a polymerase chain reaction PCR and a microarray, PCR hybridized probes are directly fixed on a hybridization cabin in the microarray and are on a same chip with a PCR reaction chamber; the detection method includes the steps of extracting a blood sample DNA liquid, carrying out PCR amplification, hybridizing, cleaning, and reading and discriminating a result. The method can quickly and sensitively detect plasmodium falciparum, can greatly improve the detection efficiency of front line inspection and quarantine personnel of import and export ports, not only can reduce the workload, but also furthest solves a positive detection missing problem possibly existing in a traditional detection method, so as to furthest prevent generation of plasmodium epidemic situation.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a rapid and sensitive detection method for Plasmodium falciparum. Background technique [0002] Malaria is one of the most serious global public health problems, and more than half of the world's population lives in malaria-endemic areas. In recent years, imported malaria in my country has been on the rise year by year. In 2011, 27 provinces (cities, districts) reported 4,479 cases, 66.4% of which were imported cases, and 33 cases died. Hunan Province reported 148 cases, mainly caused by Plasmodium falciparum (P.f) and Plasmodium vivax (P.v). At present, microscopic examination is still used as the "gold standard" for diagnosing malaria in my country. Microscopic examination is affected by the subjective judgment and sense of responsibility of microscopic personnel, and it is very easy to miss diagnosis and delay treatment. In order to improve the ability o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/90
CPCC12Q1/6837C12Q1/6893C12Q2531/113C12Q2565/501Y02A50/30
Inventor 田桢干张子龙李美王雪嵋李深伟曹敏汤林华
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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