Rapid and sensitive method for detection of Marburg virus RT-LAMP

Abstract

The invention relates to a rapid and sensitive method for detection of Marburg virus RT-LAMP. The detection method of the invention employs specific primers design, loop mediated isothermal amplification (LAMP) technique, and fluorescent visual reagent for simple and fast detection of amplification products. The method includes: specimen RNA extraction, RT-LAMP amplification, and interpretation of results. The method can conduct fast and sensitive detection of Marburg virus, uses simple equipment and operations and has less testing time. The invention can greatly improve the detection efficiency of the inspection and quarantine officers in the front line of import and export ports, and can reduce the workload, and solve the positive leak detection in the traditional detection method by the maximum, so as to maximally prevent the introduction of Marburg virus.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a rapid and sensitive Marburg virus RT-LAMP detection method. Background technique [0002] With global economic integration and trade liberalization, foreign medical vectors can quickly spread infectious diseases from one country or region to the world through advanced means of transportation and international trade, resulting in international spread. In recent years, new viral infectious diseases have frequently broken out in countries all over the world, posing a serious threat to human health and causing huge economic losses to society. For example, the Severe Acute Respiratory Syndrome (SARS) epidemic in some countries and regions in 2003, the avian influenza epidemic in my country and neighboring countries in 2004, the global H1N1 influenza epidemic in 2009, and the Ebola epidemic in Africa in 2014 have given society It has had a major impact on stabili...

AI Technical Summary

Problems solved by technology

However, the above-mentioned virus isolation needs to be carried out under the conditions of a biosafety level 4 (P4) laboratory, and it takes a long time, and the requirements for the laboratory and experimental personnel are extremely high, and it is not suitable for wide application and rapid screening at border ports.

Serum neutralization test is the main method for virus serological detection, but it also needs to be carried out in P4 laboratories

At the same time, in the detection of the epidemic in 2005, it was found that ELISA, IgM and other serological or immunological detection methods had low sensitivity and high false negative rate.

[0006] The sensitivity of the acid detection method has been unanimously recognized, but the conventional PCR operation is complicated, the equipment is huge, and the technical difficulty is relatively high, which is not conducive to on-site diagnosis at ports and on-site detection in epidemic areas

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