Integrated mini non-valve pump type capillary electrophoresis chip

Abstract

The chip is composed of two pieces of basis materials being bonded. The mini pump without valve, the in and out flow channels as well as intercrossed the electrophoresis separation channel and the electrophoresist sample channel are setup in the adjoining plane. The one end of the electrophoresis sample channel and the in flow channels are connected each other in 'T' form. The mini pump without valve is utilized as the automatic sample change unit in electrophoresist continuous measuring multiple samples. With having been measured, the former sample is discharged to the waste tank. Meanwhile the new sample is fed to the sample introduction point. The chip can be repeatable use so as to simplify the test device, shorten the continuous measuring time for multiple samples, lower the operation requirements.

Description

technical field [0001] The invention relates to a biochemical detection device, in particular to an integrated miniature valveless pump type capillary electrophoresis chip. Background technique [0002] Capillary Electrophoresis (CE for short) is a new type of separation and analysis technology combining electrophoresis and chromatography developed rapidly in the early 1980s. It has the characteristics of high analysis efficiency, fast analysis speed, less sample consumption and wide application range. , has become an important means of separation and analysis in the 1990s, and has a good application prospect in the fields of biology, chemistry, medicine, environmental protection, and food. The capillary electrophoresis chip technology developed rapidly after the 1990s is based on micro-processing technology, with crystalline silicon, glass, plastic (mainly plexiglass), ceramics and silicone rubber as the base material, and the sample is processed b...

AI Technical Summary

Problems solved by technology

[0003] (1) In the process of continuous detection of multiple samples, after the detection of one sample is completed, the capillary electrophoresis chip must be removed from the detection device, and the original sample remaining in the chip must be cleaned manually and prepared for the next one. It takes a long time for the sample to be pretreated on the chip

At the same time, when the chip is replaced with a new sample, it needs to be repositioned with the detection device. Since the size of the capillary electrophoresis tube is about tens of microns, repositioning also requires experience and a certain amount of time, and repeated positioning will also cause front and rear The repeatability of the two detections becomes poor, which affects the detection accuracy

Therefore, the use of manual sample change has an impact on the speed and accuracy of continuous detection of multiple samples, especially in the problem of taking too long

[0004] (2) At present, most capillary electrophoresis chips are disposable, there is a relatively large waste, and the detection cost is high

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