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A GQDS-based Resonant Light Scattering Probe Combined with CHA Technology for Quantitative Detection of Mutant DNA

A resonant light scattering and quantitative detection technology, which is applied in the direction of scattering characteristic measurement, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of long detection time, high detection cost, complicated detection process, etc., and achieve detection time Short, simple detection equipment, high sensitivity

Active Publication Date: 2022-05-31
XIANGTAN UNIV
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Problems solved by technology

[0004] In order to improve the problems of existing analytical techniques such as complex detection process, high detection cost, and long detection time during DNA detection, the purpose of the present invention is to provide a low-cost, high-sensitivity, high-selectivity detection method to realize DNA detection. detection

Method used

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  • A GQDS-based Resonant Light Scattering Probe Combined with CHA Technology for Quantitative Detection of Mutant DNA
  • A GQDS-based Resonant Light Scattering Probe Combined with CHA Technology for Quantitative Detection of Mutant DNA
  • A GQDS-based Resonant Light Scattering Probe Combined with CHA Technology for Quantitative Detection of Mutant DNA

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Experimental program
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Embodiment 1

[0045] As shown in Figure 5, from curve a to e, the shape of the resonant light curve is almost the same. Hence the resonance at 460nm

[0047] A 2.5% agarose gel electrophoresis experiment was used to verify the feasibility of catalyzing the hairpin self-assembly amplification reaction. sample

Embodiment 3

Embodiment 4

[0054] A series of different concentrations of p53 mutant DNA was added with the probe and the signal was obtained under optimal conditions. like

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Abstract

The invention discloses a method for quantitatively detecting mutant DNA by combining GQDs-based resonance light detection probes with CHA technology. The invention firstly synthesizes graphene quantum dots, then designs two kinds of hairpin probes H1 and H2 for the target object, and then prepares two kinds of probes GQDs‑H1 and GQDs‑H2 through condensation reaction of the probes and GQDs. In the PBS buffer solution reaction system, when p53 mutant DNA is added, the aggregation of GQDs-H1 and GQDs-H2 will be caused, and a significantly enhanced resonance light signal will be generated to realize the quantitative detection of p53 mutant DNA. The invention has high sensitivity and selectivity, the linear range is 1pM to 50nM, the detection limit is 0.8pM, and the target object can be quantitatively detected effectively.

Description

A GQDs-based Resonance Light Scattering Probe Combined with CHA Technology for Quantitative Detection of Mutation DNA method technical field The present invention relates to molecular detection field, relate in particular to a kind of based on GQDs resonance light scattering probe combined with CHA technology Methods for quantitative detection of mutant DNA. Background technique Graphene quantum dots (GQDs) have the characteristics of good photostability, good water solubility, low toxicity and good biocompatibility. Therefore, it is widely used in various fields of biomedicine. Example: Graphene quantum dots could serve as payloads for drug delivery Body (Zheng, X.T., Than, A., Ananthanaraya, A., Kim, D.H., Chen, P., Acs Nano, 2013, 7, 6278‑ 6286), graphene quantum dots also have good drug loading capacity (Wang, J., Liu, C.H., Shuai, Y., Cui.X.Y.,B.,Xia,Y.Z.,Zhang,Niel.L.,Colloids Surf.B Biointerfaces,2013,112, 192‑6). Besides, graphene quantum dots can als...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6827G01N21/47
CPCY02A50/30
Inventor 蔡昌群韩云鹏王双龚行
Owner XIANGTAN UNIV
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