Resonance light scattering detection method for sodium heparin

A technology of resonance light scattering and detection method, applied in the field of analysis, can solve the problems of complex molecular structure of heparin sodium and incapability of artificial chemical synthesis, etc., and achieve the effects of short detection time, rich detection methods, and satisfying detection requirements.

Inactive Publication Date: 2012-10-31
ARMY MEDICAL UNIV
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Problems solved by technology

[0003] The molecular structure of heparin sodium is complex, and artificial chemical synthesis is impossible in the short term. At present, only heparin sodium extracted from pig or bovine intestinal mucosa can be used for clinical treatment

Method used

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  • Resonance light scattering detection method for sodium heparin
  • Resonance light scattering detection method for sodium heparin
  • Resonance light scattering detection method for sodium heparin

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Embodiment Construction

[0024] Hereinafter, the present invention will be described in detail through specific experiments with reference to the accompanying drawings.

[0025] The heparin sodium solid used in the experiment is the product of China Pharmaceutical Group Shanghai Chemical Reagent Company (160 IU / mg), and its solution is prepared with water; the Co(II) solution is CoCl 2 aqueous solution; the cobalt reagent is 4-(5-chloro-2-pyridylazo)-1,3-diaminobenzene, abbreviated as 5-Cl-PADAB, and its solution is prepared with absolute ethanol. The resonance light scattering spectrum and intensity involved in the experiment ( I ) were measured by F-4500 fluorescence spectrophotometer (Hitachi).

[0026] 1. Resonance light scattering spectrum characteristics of the interaction between Co(II), cobalt reagent and sodium heparin

[0027] Add 1.0 mL 2.0×10 to a 10.0 mL test tube sequentially -4 mol / L Co(II) solution, 0.8 mL 5.0×10 -4 mol / L cobalt reagent solution and 1.4 mL 0.3 mol / L NaOH solutio...

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Abstract

The invention discloses a resonance light scattering detection method for sodium heparin, wherein under an alkaline condition with the NaOH concentration being 0.042mol/L, Co(II) and a cobalt reagent are bonded to produce a complex with positive charges, the sodium heparin carries negative charges and is electrostatically bonded with the complex to form a ternary complex, so that the intensity of resonance light scattering is enhanced obviously and has the maximal scattering peak located at 550nm, the scattering intensity is in good linear relation with the concentration of the sodium heparin in a range of 0.05-1.60 mu g/mL, and the detection limit (3 sigma) is 1.3ng/mL. The method has the advantages of simple required detection equipment, readily available reagent, simpleness for operation, short detection time, wider linear range and higher sensitivity. The invention enriches the detection methods of the sodium heparin and is favorable for meeting detection requirements under different conditions.

Description

technical field [0001] The invention belongs to the technical field of analysis and relates to a drug analysis method. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer of human diseases. With the overnutrition brought about by the improvement of people's living standards, the deterioration of the global environment, the accelerated pace of life, and the aging population, the incidence and mortality of cardiovascular and cerebrovascular diseases in the world are increasing year by year. Heparin sodium is currently the most effective and clinically used anticoagulant drug in the world. It is mainly used for cardiovascular and cerebrovascular diseases and hemodialysis treatment, and is the only effective specific drug in hemodialysis treatment. Clinical application and research have shown that in addition to its anticoagulant effect, heparin sodium also has a variety of other biological activities and clinical uses, including ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 武丽萍赵华文杨旭贺建李兰兰刘欢
Owner ARMY MEDICAL UNIV
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