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Detection kit and detection method for dibutyl phthalate in edible oil and fatty foods

A technology of dibutyl phthalate and detection kits, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of unfavorable rapid detection and supervision, reliance on professional and technical personnel, unfavorable grassroots quality inspection departments, etc., and achieve good business The effect of value and use value, convenient operation and low cost

Active Publication Date: 2019-05-07
BEIJING PRIMEBIOTEK COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have the advantages of high detection accuracy, low detection limit, and good repeatability, they also have problems such as expensive equipment, complicated operation, not suitable for rapid detection, and relying on professional and technical personnel, which is not conducive to the grassroots quality inspection department as a large-scale primary screening. , Not conducive to on-site rapid detection and supervision

Method used

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  • Detection kit and detection method for dibutyl phthalate in edible oil and fatty foods
  • Detection kit and detection method for dibutyl phthalate in edible oil and fatty foods
  • Detection kit and detection method for dibutyl phthalate in edible oil and fatty foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Components of the kit: including dibutyl phthalate (DBP) standard solution, solid phase carrier coated with antigen, anti-reagent, enzyme marker, chromogenic solution, stop solution, concentrated washing solution, Reconstitution solution and purifier.

[0059] The concentration of anti-reagent is 0.05mg / L;

[0060] The concentration of the enzyme label is 2mg / mL;

[0061] The antigen coating concentration of the solid phase carrier was 4mg / L.

[0062] Dibutyl phthalate standard solution is prepared from dibutyl phthalate standard product, the concentrations are 0, 0.03, 0.09, 0.27, 0.81, 2.43 mg / kg, and put into glass vials.

[0063] The solid phase carrier is composed of a microporous plate, and the microporous plate is coated with dibutyl phthalate complete antigen, and the dibutyl phthalate complete antigen is composed of dibutyl phthalate hapten and bovine serum white Obtained by protein coupling; the dibutyl phthalate hapten is prepared by reacting 4-nitroph...

Embodiment 2

[0087] (1) The composition of the kit: dibutyl phthalate standard solution, solid phase carrier coated with antigen, anti-reagent, enzyme marker, chromogenic solution, stop solution, concentrated washing solution, complex solution and purifying agent .

[0088] The concentration of anti-reagent is 0.2mg / L;

[0089] The concentration of the enzyme label is 1mg / L;

[0090] The antigen coating concentration of the solid phase carrier was 1mg / L.

[0091] Dibutyl phthalate standard solution is prepared from dibutyl phthalate standard product, the concentrations are 0, 0.03, 0.09, 0.27, 0.81, 2.43 mg / kg, and put into glass vials.

[0092] The solid phase carrier is composed of a microporous plate, and the microporous plate is coated with dibutyl phthalate complete antigen, and the dibutyl phthalate complete antigen is composed of dibutyl phthalate hapten and chicken egg white Obtained by protein coupling; the dibutyl phthalate hapten is prepared by reacting 4-nitrophthalic anhydr...

Embodiment 3

[0112] (1) The composition of the kit: dibutyl phthalate standard solution, solid phase carrier coated with antigen, anti-reagent, enzyme marker, chromogenic solution, stop solution, concentrated washing solution, complex solution and purifying agent .

[0113] The concentration of anti-reagent is 1mg / L;

[0114] The concentration of the enzyme label is 0.5mg / L;

[0115] The antigen coating concentration of the solid phase carrier is 0.2mg / L.

[0116] Dibutyl phthalate standard solution is prepared from dibutyl phthalate standard product, the concentrations are 0, 0.03, 0.09, 0.27, 0.81, 2.43 mg / kg, and put into glass vials.

[0117] The solid phase carrier is composed of a microporous plate, and the microporous plate is coated with dibutyl phthalate complete antigen, and the dibutyl phthalate complete antigen is composed of dibutyl phthalate hapten and mouse serum albumin Coupling obtained; the dibutyl phthalate hapten is prepared by reacting 4-nitrophthalic anhydride with...

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Abstract

The invention provides a detection kit and a detection method for dibutyl phthalate in edible oil and fatty foods. The detection kit comprises a dibutyl phthalate standard solution, a solid phase carrier coated with an antigen, an anti-reagent, an enzyme marker, a color developing solution, a stop solution, a concentrated washing solution, a complex solution, and a purifying agent. The detection method comprises a sample preparation step and a detection step. The kit and the method can directly detect the dibutyl phthalate in the edible oil and the fatty foods, and has the advantages of simpledetection device, high detection sensitivity, low cost, convenient operation, and convenient carrying, so that the industrial production can be realized.

Description

technical field [0001] The invention belongs to the field of detection kits, in particular to a detection kit and a detection method for dibutyl phthalate in edible oil and fat-containing foods. Background technique [0002] The analysis methods of dibutyl phthalate in edible oil and fat-containing foods include liquid chromatography, gas chromatography, gas chromatography, etc. Although these methods have the advantages of high detection accuracy, low detection limit, and good repeatability, they also have problems such as expensive equipment, complicated operation, unsuitability for rapid detection, and reliance on professional and technical personnel, which is not conducive to primary screening by grassroots quality inspection departments. , It is not conducive to on-site rapid detection and supervision. Contents of the invention [0003] In view of this, the present invention aims to propose a detection kit and a detection method for dibutyl phthalate in edible oil an...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 邓乾民张翊孙清王莉
Owner BEIJING PRIMEBIOTEK COMPANY
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