Ratio fluorescent probe, fluorescent paper chip and detection method for detecting mercury ions

Abstract

The invention discloses a ratio fluorescent probe, a fluorescent paper chip and a detection method for detecting mercury ions, the ratio fluorescent probe comprises a background probe and a reaction probe, the background probe is composed of nitrogen-doped blue carbon quantum dots, the reaction probe is composed of red gold nanoclusters, the ratiometric fluorescent probe is formed by mixing red gold nanoclusters and nitrogen-doped blue carbon quantum dots, after mercury ions are added into the ratiometric fluorescent probe, the red fluorescence of the gold nanoclusters is gradually quenched, and the fluorescence of the nitrogen-doped blue carbon quantum dots is kept unchanged and is used as a reference signal; and therefore, the On-Off type fluorescent probe is formed. The two fluorescent materials of the ratiometric fluorescent probe are easy to prepare, and the ratiometric fluorescent probe has the characteristics of low toxicity, high fluorescence intensity, good biocompatibility and the like, has a wider color change range, and is more beneficial to detection of mercury ions. When the probe is fixed on a paper chip sensor, rapid visual detection of mercury ions can be realized.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and more specifically relates to a ratio fluorescent probe for detecting mercury ions, a fluorescent paper chip and a detection method. Background technique [0002] Heavy metal ions are environmental pollutants that can cause serious harm to human health, especially mercury(II) ions (Hg 2 + ). Unlike organic pollutants, Hg 2+ It is difficult to decompose through biodegradation, and may continue to accumulate in organisms and enter the food chain, thereby causing serious harm to organisms and humans. In particular, highly toxic mercury vapors and mercury salts (except for some with extremely low solubility such as mercury sulfide) may cause adverse effects on human health even at ultra-trace levels due to their adverse effects on living organs and tissues. long-term irreversible damage. Therefore, how to detect the content of mercury ions simply, quickly and sensitively has att...

AI Technical Summary

Problems solved by technology

Although these methods have high detection sensitivity and accurate detection results, in most cases can provide good detection of Hg 2+ However, due to factors such as expensive instruments, complicated sample processing procedures, and high analysis costs, this type of method is limited to a certain extent to be used in laboratories

In order to make the detection more convenient and flexible, many scholars at home and abroad have researched and developed many methods and devices for quickly detecting mercury ions, mainly including disposable mercury ion immunosensors (CN110514730A), fluorescent molecular probes (CN111533692A), paper chips (CN111474153A, CN111208100A, CN111474153A), paper-based microfluidic chip (CN110898866A), fluorescent test paper method (CN210604398U, CN110596084A, CN110646606A), fluorescence / electrochemical sensor (CN110618272A), surface-enhanced Raman sensor (CN1101860A biosensor902A (CN110186902A), electrochemical sensor , CN110907518B), hydrogel (CN110079584A, CN109900692A), two-photon fluorescent probe (CN110423610A), enzymatic reaction double emission fluorescent probe (CN110987881A), rare earth / nucleotide supramolecular assembly (CN110823851A), nucleic acid protein complex Compared with traditional detection methods, most of the rapid detection methods have the advantages of low cost, fast detection speed, portability and simple operation, but their accuracy and sensitivity are low.

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