Adipocyte protective solution and preparation method thereof

A fat cell and protective solution technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of difficult clinical application, harsh use conditions, low cell survival rate, etc., to protect adipose stem cells and achieve stable performance. , the effect of enhancing the reproductive capacity

Inactive Publication Date: 2017-09-01
CENTURY BIOSTRENGTH BEIJING PTY LTD
6 Cites 21 Cited by

AI-Extracted Technical Summary

Problems solved by technology

The cell protection solutions disclosed in the prior art generally have problems such as low cell survival rate...
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Method used

As can be seen from Fig. 1, the proliferation rate of the fat stem cells preserved by the fat cell protection solution of Example 19 is significantly higher than the fat stem cells preserved by the fat cell protection solution of Example 4, Comparative Example 13 and Comparative Example 14 , the above results show that adding trehalose, hydroxypropyl cyclodextrin and sodium alginate to the fat cell protection solution can effectively increase the proliferation rate of adipose stem cells, reduce one of the components, or change one of the components to increase the proliferation rate of adipose stem cells effect will be weakened.
Comparing the fungus and virus infection status of the adipocyte protection liquid of embodiment 7 and embodiment 4, comparative example 5-6, adding benzalkonium chloride and calcium formate in the adipocyte protection liquid can effectively prevent adipocyte protection If the liquid is infected with fungi and viruses, if one of the components is reduced, or one of the components is changed, the effect of the fat cell protection liquid on preventing fungal and viral infections will be weakened. Analysis of the effect of hypoxic protective agents on the survival rate of adipose stem cells under hypoxic conditions:
Comparing the stability situation of the adipocyte protection liquid of embodiment 22 and embod...
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Abstract

The invention provides an adipocyte protective solution. The protective solution is mainly prepared by dissolving a low-oxygen protective agent, vitamin C and aminoglycoside antibiotics with an aqueous solution of DMEM/F12 medium. Each 1 mL of the aqueous solution of DMEM/F12 medium is respectively used for dissolving 5-10 mg of the low-oxygen protective agent, 10-50 micrograms of the vitamin C and 50-200 U of the aminoglycoside antibiotics. Concentration of the aqueous solution of DMEM/F12 medium is 30-40 mg/mL. The adipocyte protective solution has stable performance, can preserve adipocyte for a long time, has effects of effectively keeping activity of adipose-derived stem cells and protecting adipose-derived stem cells, can enhance proliferation capacity of adipose-derived stem cells, can meet the requirement of transporting adipocyte to most areas of the country and surrounding countries, is safe and nontoxic, and can be directly applied to human body.

Application Domain

Technology Topic

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  • Adipocyte protective solution and preparation method thereof
  • Adipocyte protective solution and preparation method thereof
  • Adipocyte protective solution and preparation method thereof

Examples

  • Experimental program(24)
  • Comparison scheme(16)
  • Effect test(1)

Example Embodiment

[0034] Example 1
[0035] An adipocyte protective solution, which is mainly prepared by dissolving hypoxia protective agent, vitamin C and gentamicin in DMEM/F12 medium aqueous solution, and each mL of the DMEM/F12 medium aqueous solution separately dissolves the Hypoxia protective agent 8 mg, the vitamin C 20 μg, the gentamicin 100 U, and the concentration of the DMEM/F12 medium aqueous solution is 37.5 mg/mL.

Example Embodiment

[0036] Example 2
[0037] An adipocyte protective solution, which is mainly prepared by dissolving hypoxia protective agent, vitamin C and gentamicin in DMEM/F12 medium aqueous solution, and each mL of the DMEM/F12 medium aqueous solution separately dissolves the Hypoxia protective agent 5 mg, the vitamin C 10 μg, the gentamicin 50 U, and the concentration of the DMEM/F12 medium aqueous solution is 30 mg/mL.

Example Embodiment

[0038] Example 3
[0039] An adipocyte protective solution, which is mainly prepared by dissolving hypoxia protective agent, vitamin C and gentamicin in DMEM/F12 medium aqueous solution, and each mL of the DMEM/F12 medium aqueous solution separately dissolves the Hypoxia protective agent 10mg, the vitamin C 50μg, the gentamicin 200U, the concentration of the DMEM/F12 medium aqueous solution is 40mg/mL.
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PUM

PropertyMeasurementUnit
Concentration37.5mg/ml
tensileMPa
Particle sizePa
strength10

Description & Claims & Application Information

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Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
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