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Adipocyte protective solution and preparation method thereof

A fat cell and protective solution technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of difficult clinical application, harsh use conditions, low cell survival rate, etc., to protect adipose stem cells and achieve stable performance. , the effect of enhancing the reproductive capacity

Inactive Publication Date: 2017-09-01
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cell protection solutions disclosed in the prior art generally have problems such as low cell survival rate, poor safety, and relatively harsh use conditions, and there are certain difficulties in clinical application.

Method used

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  • Adipocyte protective solution and preparation method thereof
  • Adipocyte protective solution and preparation method thereof
  • Adipocyte protective solution and preparation method thereof

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Effect test

Embodiment 1

[0035] A kind of adipocyte protection liquid, this protection liquid is mainly that hypoxia protection agent, vitamin C and gentamycin are dissolved with DMEM / F12 medium aqueous solution, and each mL of described DMEM / F12 medium aqueous solution dissolves respectively described Hypoxia protective agent 8 mg, vitamin C 20 μg, gentamicin 100 U, and the concentration of the DMEM / F12 medium aqueous solution is 37.5 mg / mL.

Embodiment 2

[0037] A kind of adipocyte protection liquid, this protection liquid is mainly that hypoxia protection agent, vitamin C and gentamycin are dissolved with DMEM / F12 medium aqueous solution, and each mL of described DMEM / F12 medium aqueous solution dissolves respectively described Hypoxia protective agent 5 mg, vitamin C 10 μg, gentamicin 50 U, and the concentration of the DMEM / F12 medium aqueous solution is 30 mg / mL.

Embodiment 3

[0039] A kind of adipocyte protection liquid, this protection liquid is mainly that hypoxia protection agent, vitamin C and gentamycin are dissolved with DMEM / F12 medium aqueous solution, and each mL of described DMEM / F12 medium aqueous solution dissolves respectively described Hypoxia protective agent 10 mg, vitamin C 50 μg, gentamicin 200 U, and the concentration of the DMEM / F12 medium aqueous solution is 40 mg / mL.

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Abstract

The invention provides an adipocyte protective solution. The protective solution is mainly prepared by dissolving a low-oxygen protective agent, vitamin C and aminoglycoside antibiotics with an aqueous solution of DMEM / F12 medium. Each 1 mL of the aqueous solution of DMEM / F12 medium is respectively used for dissolving 5-10 mg of the low-oxygen protective agent, 10-50 micrograms of the vitamin C and 50-200 U of the aminoglycoside antibiotics. Concentration of the aqueous solution of DMEM / F12 medium is 30-40 mg / mL. The adipocyte protective solution has stable performance, can preserve adipocyte for a long time, has effects of effectively keeping activity of adipose-derived stem cells and protecting adipose-derived stem cells, can enhance proliferation capacity of adipose-derived stem cells, can meet the requirement of transporting adipocyte to most areas of the country and surrounding countries, is safe and nontoxic, and can be directly applied to human body.

Description

technical field [0001] The invention belongs to the technical field of fat cell preservation, and in particular relates to a fat cell protection solution and a preparation method thereof. Background technique [0002] In recent years, cell technology has been widely used in clinical trials and treatment of various diseases, and has shown good application prospects, and has gradually become one of the new medical research hotspots in recent years. Adipocytes can proliferate stably in vitro and have a low rate of death. They have the advantages of being easy to obtain materials, not limited to age and time, a large number of cells can be obtained from a small amount of tissue, suitable for large-scale culture, and less damage to the body. Therefore, it is particularly important to study an effective method of adipocyte preservation, so that adipocytes with clinical application value can be preserved for a long time in vitro and maintain cell viability. [0003] At present, th...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李霞云曹毓琳白志惠刘世红卢承前
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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