A method for identifying the backfat thickness of living pigs and a genetic diagnostic kit
A technology for thick backfat and pig identification, applied in the field of genetic engineering, can solve the problems of single flavor and deterioration of pork quality.
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[0079] Embodiment 1, a method for assisting in identifying the backfat thickness of live pigs with a body weight of 100 kg
[0080] 1. Screening of SNP sites of porcine MEG3 gene
[0081] 1. Extraction of DNA from pig ear tissue samples
[0082] The blood samples of 32 Landrace pigs from Hebei pig farms were collected respectively. After the samples were collected, they were stored in 75% alcohol and stored at -20°C. The genomic DNA of blood samples was extracted by phenol-chloroform extraction, and the specific steps were as follows:
[0083] (1) Preparation of ear tissue samples: Cut ear tissue samples of moderate size, wash away impurities and blood stains on the surface with normal saline, put them in a 1.5ml centrifuge tube, cut them into pieces with ophthalmic scissors, and add 500uL of tissue to it Lysis solution (Beijing Baitaike Biotechnology Co., Ltd.; product number: AU19011);
[0084] (2) According to the size of the cut ear tissue sample, add 15-25uL proteinase...
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