A control method for the vertical transmission of microparticle disease embryos in double-crossed silkworm stock

A microparticle disease and silkworm technology, applied in animal husbandry, etc., can solve the problems of lack of treatment methods, misjudgment and missed judgment, and achieve the effect of relieving silkworm egg diapause and improving hatching rate

Active Publication Date: 2020-04-14
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of specific treatment methods for silkworm microsomosis. At present, it is mainly to check the microsporidia of ovipositing moths under a microscope, and remove the poisonous silkworm eggs according to the results of the microscopic examination, so as to prevent and control the germ infection of silkworm microsomosis.
However, this method is related to the experience and technical level of the drug inspectors, and it is easy to cause misjudgment and missed judgment.

Method used

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  • A control method for the vertical transmission of microparticle disease embryos in double-crossed silkworm stock
  • A control method for the vertical transmission of microparticle disease embryos in double-crossed silkworm stock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preventing and controlling embryo seed infection of silkworm double-cross original seed microparticle disease, comprising the following steps:

[0025] "Liangguang No. 2" double-cross original species, after laying eggs, protect the silkworm eggs at 25°C for 20 hours, and then put them in 47°C with a density of 1.075g / cm 3 Immerse in hydrochloric acid solution for 10 minutes, then take it out for elution, dry, green and hatch. The practical hatching rate is up to 94%, and the relative control effect is up to 95% compared with the untreated control under microscope inspection of the 3rd instar silkworm.

[0026] The formula for calculating the relative control effect is as follows:

[0027] Relative control effect (%)=(100-treatment group morbidity / control group morbidity)×100

Embodiment 2

[0029] A method for preventing and controlling embryo seed infection of silkworm double-cross original seed microparticle disease, comprising the following steps:

[0030] "Liangguang No. 2" double-cross original species, after laying eggs, protect the silkworm eggs at 25°C for 20 hours, and then put them in 48°C with a density of 1.075g / cm 3 Immerse in hydrochloric acid solution for 6 minutes, then take it out for elution, dry, green and hatch. The practical hatching rate is up to 93%, and the relative control effect is up to 96% compared with the untreated control under the microscope inspection of the 3rd instar silkworm.

Embodiment 3

[0032] A method for preventing and controlling embryo seed infection of silkworm double-cross original seed microparticle disease, comprising the following steps:

[0033] "Liangguang No. 2" double-cross original species, after laying eggs, protect the silkworm eggs at 25°C for 20 hours, and then put them in 47.5°C with a density of 1.075g / cm 3 Immerse in hydrochloric acid solution for 8 minutes, then take it out for elution, dry, green and hatch. The practical hatching rate is up to 94%, and the relative control effect is up to 97% compared with the untreated control under microscope inspection of the 3rd instar silkworm.

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Abstract

The invention discloses a control method for semina vertical transmission of bombyx mori double cross protospecies pebrine. The method comprises the following steps that for silkworm eggs free of refrigeration, after bombyx mori produces the silkworm eggs, the silkworm eggs are kept staying at 20-30 DEG C for 15-24 h, and soaked in water at 47-48 DEG C for 6-10 min, and then the silkworm eggs are taken out, washed until no smells exists, and dried; for refrigerated silkworm eggs, after the refrigerated silkworm eggs are taken out from a refrigerator, the refrigerated silkworm eggs are kept staying at 15-20 DEG C for several hours for a buffer effect, put into water at 47-50 DEG C for soaking for 6-10 min, and the refrigerated silkworm eggs are taken out, washed until no smells exists, and dried. By means of the control method for semina vertical transmission of bombyx mori double cross protospecies pebrine, the relative control rate of semina vertical transmission of bombyx mori pebrine can reach 90-97%, the hatching rate of the silkworm eggs can reach more than 92%, and the control method for semina vertical transmission of bombyx mori double cross protospecies pebrine has no harmful effects on the growth of the bombyx mori.

Description

technical field [0001] The invention belongs to the field of sericulture production, and in particular relates to a method for preventing and controlling the vertical transmission of microparticle disease embryos of silkworm double-crossed original seeds. Background technique [0002] Bombyx mori microsporidia is an infectious silkworm disease caused by pathogenic microsporidia such as Nosema bombycis infecting silkworms. The disease can be infected by eating mulberry leaves, and can also be infected by embryo seeds, so the disease has always been valued by the industry and is the only object of silkworm eggs quarantine. [0003] In the 1980s and 1990s, my country's sericulture developed rapidly, but the backward production facilities of silkworm eggs and extensive technical management led to the epidemic of silkworm microparticle disease in the whole country. In recent years, with the change of sericulture area, seed production environment and business model, silkworm micr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 杨琼邢东旭廖森泰肖阳李庆荣赵超艺叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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