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Prevention and control methods for the vertical transmission of microparticle disease embryos in four-element hybrids Bombyx mori

A technology of silkworm microparticles and vertical transmission, which is applied in animal husbandry and other fields, can solve problems affecting the yield and quality of silkworm eggs, toxic and side effects of silkworms, etc., and achieve the effect of relieving diapause of silkworm eggs and improving hatching rate

Active Publication Date: 2018-06-19
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that long-term use of drugs can cause toxic and side effects to silkworms, affecting the yield and quality of silkworm eggs

Method used

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  • Prevention and control methods for the vertical transmission of microparticle disease embryos in four-element hybrids Bombyx mori
  • Prevention and control methods for the vertical transmission of microparticle disease embryos in four-element hybrids Bombyx mori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0024] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 47°C for 10 minutes, then taken out, eluted, dried, accelerated greening, and hatched. The practical hatching rate is up to 98%. Microscopic examination of the third instar silkworm shows that the incidence rate is only 7.44%, while the incidence rate in the control group is as high as 83.12%. Compared with the untreated control group, the relative control effect is 91%.

[0025] The formula for calculating the relative control effect is as follows:

[0026] Relative control effect (%)=(100-treatment group morbidity / control group morbidity)×100

[0027] Real-time PCR was used to quantitatively detect the pathogen of Microsporidium ...

Embodiment 2

[0035] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0036] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 48°C for 6 minutes, then removed, eluted, dried, accelerated and hatched. The practical hatching rate is up to 96%. Microscopic examination of the third-instar silkworm shows that the incidence rate is only 2.65%, while the incidence rate in the control group is as high as 82.84%. Compared with the untreated control group, the relative control effect is 97%.

[0037] In addition, through Real-time PCR, quantitative detection of the pathogen of No. silkworm in the ant silkworm was carried out, and it was found that the inactivation rate of the pathogen reached 99%.

Embodiment 3

[0039] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0040] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 47.5°C for 7 minutes, then removed, eluted, dried, accelerated and hatched. The practical hatching rate is up to 99%. Microscopic examination of the third instar silkworm shows that the incidence rate is only 3.70%, while the incidence rate in the control group is as high as 83.15%, and the relative control effect is 96%.

[0041] In addition, through Real-time PCR, quantitative detection of the pathogen of No. silkworm in the ant silkworm was carried out, and it was found that the inactivation rate of the pathogen reached 97%.

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Abstract

A control method for vertical transmission of tetra hybrid bombyx mori pebrine germ. The method comprises the following steps: for silkworm eggs not needing to be refrigerated: after bombyx mori lays eggs, making the silkworm eggs stand at 20-30°C for 15-30 h, immersing the silkworm eggs in water at 47-48°C for 6-10 min, taking out the silkworm eggs and then eluting the silkworm eggs with clear water stage by stage until the silkworm eggs are odorless, and hatching and incubating the silkworm eggs after drying; for refrigerated silkworm eggs: taking the silkworm eggs out of a cold storage, protecting the silkworm eggs at 10-20°C for several hours, putting the silkworm eggs in water at 47-52°C for immersion for 6-10 min, taking out the silkworm eggs and then eluting the silkworm eggs with clear water stage by stage until the silkworm eggs are odorless, and hatching and incubating the silkworm eggs after drying. The method can inactivate 97-99% of tetra hybrid bombyx mori sporozoan pathogeny, achieves a relative control effect up to 90-97% on vertical transmission of bombyx mori pebrine germ, obtains silkworm egg hatchability up to 93%, and causes no harmful effect on growth and development of bombyx mori.

Description

technical field [0001] The invention belongs to the field of sericulture production, and in particular relates to a method for preventing and controlling the vertical transmission of microparticle disease embryos of four-element hybrid silkworm silkworm. Background technique [0002] Bombyx mori microparticle disease is the most serious devastating infectious disease in sericulture production. The pathogen of the disease is Nosema bombycis, which is a typical species of Nosema genus. Microsporidium silkworm can parasitize in silkworm midgut, silk gland, muscle, Malpighian duct and other tissues. After infection, the silkworm is inactive, stunted, and dark brown spots appear on the body surface, eventually leading to the death of the silkworm. [0003] Microsporidium silkworm can not only infect silkworms by eating mulberry leaves, but also can be transmitted through silkworm eggs. Therefore, the disease has always been valued by the industry, and it has been listed as the on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 邢东旭杨琼廖森泰李庆荣肖阳赵超艺叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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