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Universal molecular marker primers for trees and their joint development methods and applications

A technology of molecular markers and trees, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve problems such as the inability to extract intron sequences

Active Publication Date: 2021-05-14
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0009] PIP markers are suitable for species that only have published genome sequences but no specific genome structure information. Since there is no detailed intron site information, it is impossible to extract intron sequences according to conventional methods

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  • Universal molecular marker primers for trees and their joint development methods and applications
  • Universal molecular marker primers for trees and their joint development methods and applications
  • Universal molecular marker primers for trees and their joint development methods and applications

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0034] refer to figure 1 , the joint development method of the SSR of tree of the present invention, ILP and PIP universal molecular marker primer, comprises the following steps:

[0035] Step1: SSR site and intron site scanning

[0036] Download all tree genome sequences and genome annotation information from the public data platform, scan the SSR site, intron site and predicted intron site in the genome sequence, and obtain the corresponding site information.

[0037] It is generally believed that the minimum length of an SSR site is 12bp, that is, a single nucleotide repeat unit is at least 12 repeats, a dinucleotide repeat unit is at least 6 repeats, a trinucleotide repeat unit is at least 4 repeats, and a tetranucleotide repeat is at least 4 repeats. The unit is at least 3 repeats, the pentanucleotide repeating unit is at least 3 repea...

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Abstract

The invention discloses a general molecular marker primer for trees and its joint development method and application. The invention adopts the joint development method of three molecular markers, which avoids the limitation of a single molecular marker, such as the sequence species on both sides of the SSR sequence. The low conservation between SSR markers greatly limits the utilization of SSR markers in other species. ILP markers and IP are only present in chromosome gene segments, and this method cannot be used for other non-coding regions. The combined use of multiple molecular marker methods Combining the advantages of each marker, the result is more objective; the SSR, ILP and PIP universal molecular marker primers of trees obtained by the method of the present invention not only overcome the lack of molecular markers in trees, the lower versatility of markers, and the lack of tree species. It is difficult to distinguish the problem, and it can also improve the accuracy of tree species identification results.

Description

technical field [0001] The present invention relates to molecular marker primers and development methods and applications thereof, in particular to SSR, ILP and PIP universal molecular marker primers of trees, a method for jointly developing the aforementioned universal molecular marker primers based on tree genomes, and the use of the aforementioned universal molecular marker primers in trees The application belongs to the technical field of molecular marker development and application. Background technique [0002] Trees are important natural resources. On the one hand, trees play a huge role in protecting our natural ecological environment. On the other hand, trees are important furniture and industrial raw materials, and have important economic value. [0003] There is a shortage of deep forest resources in my country, uneven distribution between north and south, and low economic benefits. Breeding fine varieties is an effective way to solve the above problems. [0004]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 杨龙桑亚林夏信瑶栾琳琳
Owner SHANDONG AGRICULTURAL UNIVERSITY
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