Application of TM9SF1 gene as target point to vascular disease

A gene and target technology, applied in the application field of vascular diseases

Active Publication Date: 2017-12-01
湖北汇智铭传生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As for the relationship between endothelial cell function and TM9SF1 gene, there are even rare reports

Method used

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  • Application of TM9SF1 gene as target point to vascular disease
  • Application of TM9SF1 gene as target point to vascular disease
  • Application of TM9SF1 gene as target point to vascular disease

Examples

Experimental program
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Effect test

Embodiment 1

[0087] 1 Materials and methods

[0088] 1.1 Cells and main reagents

[0089] HUVEC were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. TM9SF1-specific siRNA (SEQ ID NO.1) was designed and synthesized by Gemma. Endothelial cell-specific medium EGM was produced by Swiss LONZA Company; trypsin (including EDTA), fetal bovine serum (FBS), and phosphate buffered saline (PBS) were produced by American Hyclone Company; transfection reagent Lipofectamine 3000 was purchased from American Thermo Company ; CD31 antibody was purchased from Immunoway Company in the United States; Immunocytochemistry Chromogenic Kit was purchased from Beijing Zhongshan Jinqiao Company; CYBR Green Mix was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; CCK8 was purchased from Yisheng Company.

[0090] 1.2 HUVEC cell culture and identification

[0091] HUVEC were cultured in EGM, a special medium for endothelial cells, at 37.5°C, 5% CO 2 Culture in an incubator wi...

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Abstract

The invention discloses an application of a TM9SF1 gene as a target point to a vascular disease, and relates to the technical field of biology. After an interference endogenous TM9SF1 gene is discovered by an RNA (ribonucleic acid) interference strategy, expression of two important genes IL1beta and IL8 related to HUVEC (human umbilical vein endothelial cell) inflammation and a gene ACE1 closely related to vascular contraction is obviously reduced, the up-regulation function for expression of the TM9SF1 gene, the IL1beta, the IL8 and the gene ACE1 is prompted, expression of the IL1beta, the IL8 and the gene ACE1 can be inhibited or silenced by inhibiting or silencing expression of the TM9SF1 gene, and the vascular disease related to the expression level of the IL1beta, the IL8 and the gene ACE1 can be treated or prevented.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of TM9SF1 gene as a target in vascular diseases. Background technique [0002] Endothelial cells are a single layer of cells located on the inner wall of blood vessels, which play a key role in the normal function of blood vessels, and their dysfunction is often closely related to vascular diseases. [0003] Nine-transmembrane superfamily protein 1 (TM9SF1, transmembrane 9superfamily protein member1) is an evolutionarily conserved nine-transmembrane protein expressed in human tissues and various cell lines. TM9SF1 protein is expressed by TM9SF1 gene, and there are few studies on TM9SF1 protein and TM9SF1 gene, especially the functional studies. As for the relationship between endothelial cell function and TM9SF1 gene, there are even rare reports. Contents of the invention [0004] The first object of the present invention is to provide the application of TM9SF1 ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K31/7105A61P29/00A61P35/00A61P9/12
CPCA61K31/7105C12Q1/6883C12Q2600/136C12Q2600/158C12N15/1138C12N2310/14G01N2800/32
Inventor 肖娟毛春何小明张国英裴素君黄纯周俊候桂郭艳华邓文彬
Owner 湖北汇智铭传生物科技股份有限公司
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