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A device and method for improving the contrast of single-molecule optical imaging

An optical imaging, single-molecule technology, applied in the optical field, can solve the problem of signal noise limitation of single-molecule fluorescence imaging, and achieve the effect of improving the contrast of single-molecule optical imaging

Active Publication Date: 2020-04-17
SHANXI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the existing photon counting technology, and to provide a device and method for improving the contrast of single-molecule optical imaging, which utilizes the quantum coherence of ultrafast laser pulses with relative delay and phase control to interact with single molecules effect, to solve the technical problems of current single-molecule fluorescence fluctuations and environmental factors that limit the signal-to-noise ratio of single-molecule fluorescence imaging

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  • A device and method for improving the contrast of single-molecule optical imaging
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  • A device and method for improving the contrast of single-molecule optical imaging

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[0023] The present invention will be further described in detail with reference to the accompanying drawings and embodiments.

[0024] Such as figure 1 As shown, a device for improving the contrast of single-molecule optical imaging, which includes a laser pulse delay and phase modulation part, a signal analysis and control part, a single-molecule excitation and a fluorescence detection part;

[0025] The laser pulse delay and phase modulation part includes a sub-picosecond pulse laser 1, a λ / 2 wave plate 2, a 50 / 50 beam splitter 3, a right-angle mirror I 4, a right-angle mirror II 5, an electro-optic modulator 6, a signal Generator 7, high-voltage amplifier 8, and λ / 4 wave plate 9; the λ / 2 wave plate 2, 50 / 50 beam splitter 3, and right-angle mirror 1 4 are successively arranged on the horizontal linearly polarized light output of sub-picosecond pulse laser 1 direction, the right-angle reflector II 5 is set in the direction of the reflected light of the 50 / 50 beam splitter 3 ...

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Abstract

The invention relates to a device and a method for increasing contrast ratio of monomolecular optical imaging and belongs to the technical field of optics. The invention mainly aims to solve the technical problem of limited signal to noise ratio of the monomolecular optical imaging caused by monomolecular fluorescence fluctuation and environmental factors at present. According to the technical scheme provided by the invention, the device for increasing contrast ratio of monomolecular optical imaging comprises a laser pulse delaying and phase modulation part, a signal analysis and control part and a monomolecular stimulating and fluorescence detection part, wherein the laser pulse delaying and phase modulation part comprises a subpicosecond pulse laser, a lambda / 2 wave plate, a 50 / 50 beam splitter, a corner cube mirror I, a corner cube mirror II, an electrooptical modulator, a signal generator, a high pressure amplifier and a lambda / 4 wave plate. Compared with the prior art, the device has the advantages of simple operation, high working efficiency, and the like.

Description

technical field [0001] The invention belongs to the field of optical technology, in particular to a device and method for improving the contrast of single-molecule optical imaging. [0002] technical background [0003] Single-molecule fluorescence imaging has become an important research method in physics, chemistry, material science, life science and many interdisciplinary subjects. The super-resolution imaging technology developed based on single-molecule fluorescence imaging has been widely used in the direct observation of life processes at the subcellular level. Since single-molecule detection eliminates the ensemble averaging effect, it can reflect local organization and molecular-scale dynamic changes, and has gradually become a powerful tool for studying the inhomogeneity of complex systems. [0004] Single-molecule fluorescence imaging mainly relies on the means of counting single-molecule fluorescent photons. However, due to single-molecule fluorescence fluctuati...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/64
Inventor 陈瑞云周海涛肖连团张国峰秦成兵高岩贾锁堂
Owner SHANXI UNIV
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