A kind of method utilizing the allelopathic effect of calamus calamus to regulate the rooting of foxtail algae
A technology of foxtail algae and calamus, applied in the biological field, can solve the problems of high cost of hormone drugs, difficult operation, and high culture conditions, and achieve the effect of accelerating reproduction.
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Embodiment 1
[0024] 1) Dig the rhizomes of the yellow calamus, wash them with water, dry them in the shade, crush them with a grinder, and pass them through a 100-mesh sieve.
[0025] 2) Accurately weigh the yellow calamus rhizome powder and deionized water and mix them in a weight ratio of 1:1000, put them in a triangular flask, and mix well. Put it into a light incubator at 25°C and continue to extract for 48 hours, shaking for 10 minutes every 12 hours.
[0026] 3) After the extraction is completed, the obtained extract is poured into a centrifuge tube, centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and filtered to obtain the yellow calamus rhizome extract.
[0027] 4) Select the Myriophyllum sp. plants that are growing well and have no pests and diseases, and cut the stem segments 8-10cm away from the stem tip as explants. The length of the Myriophyllum explants is 8-10 cm, cleaned, and explanted. Cut off the leaves at the bottom of the body.
[0028] 5) Select explants of ...
Embodiment 2
[0032] 1) Dig the rhizomes of yellow calamus, wash them with water, and dry them in the shade. Use a grinder to grind and pass through a 200-mesh sieve.
[0033] 2) Accurately weigh the yellow calamus rhizome powder and deionized water at a weight ratio of 1:800 and mix them into a triangular flask. Mix well. Put it into a light incubator at 25°C and continue to extract for 40 hours, shaking for 15 minutes every 10 hours.
[0034] 3) After 48 hours, pour the extract into a centrifuge tube and centrifuge at 10,000 rpm for 6 minutes. Take the supernatant and filter.
[0035] 4) Select the Myriophyllum sp. plants that are growing well and have no pests and diseases, and cut the stems at a distance of 8-10 cm from the stem tip as explants. The length of the Myriophyllum explants is 8-10 cm. Clean them and cut off the bottom leaves of the explants.
[0036] 5) Select explants of the same thickness and put them into 50 mL Erlenmeyer flasks containing yellow calamus rhizome extract, and ...
Embodiment 3
[0040] 1) Dig the rhizomes of the yellow calamus, wash them with water, dry them in the shade, crush them with a grinder, and pass them through a 100 mesh sieve.
[0041] 2) Accurately weigh the yellow calamus rhizome powder and deionized water and mix them at a weight ratio of 1:600, put them in a triangular flask, and mix well. Put it into a light incubator at 25°C and continue to extract for 48 hours, shaking for 10 minutes every 12 hours.
[0042] 3) After the extraction is completed, the obtained extract is poured into a centrifuge tube, centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and filtered to obtain the yellow calamus rhizome extract.
[0043] 4) Select the Myriophyllum sp. plants that are growing well and have no pests and diseases, and cut the stem segments 8-10cm away from the stem tip as explants. The length of the Myriophyllum explants is 8-10 cm, cleaned, and explanted. Cut off the leaves at the bottom of the body.
[0044] 5) Select explants of t...
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