Composition for discriminating deer species through GeXP multiple PCR method, kit and method thereof

A composition and multiple technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc., can solve the problem of limited flux amplification preference, shorten the detection cycle and avoid preference , the effect of improving the detection efficiency

Active Publication Date: 2018-03-16
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This technology solves the problems of limited throughput and amplification preference in other technologies

Method used

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  • Composition for discriminating deer species through GeXP multiple PCR method, kit and method thereof
  • Composition for discriminating deer species through GeXP multiple PCR method, kit and method thereof
  • Composition for discriminating deer species through GeXP multiple PCR method, kit and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In this example, the specificity, sensitivity and specificity cross-reactivity of primers for red deer, reindeer, elk, fallow deer and white-lipped deer were verified.

[0026] GeXP multiplex PCR specific chimeric primer sequences were synthesized by Invitrogen Company in the United States. These 5 pairs of specific chimeric primers were respectively based on the original specific primers, and the 5' ends of the upstream and downstream primers were connected to a universal tag. The length of the amplified fragments of the five deer species was increased by 37 bp. The specific sequence is shown in Table 1 below.

[0027] Table 1 GeXP-specific chimeric primers and related information

[0028]

[0029] Note: The underline indicates the base sequence of the universal tag.

[0030] 1. Gene template extraction:

[0031] DNA extraction from deer hair samples: extract DNA from deer hair samples containing hair follicles by rapid extraction method, lysate = 1×Ex Taq Buf...

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Abstract

The invention relates to a GeXP multiple PCR method for discriminating five deer of red deer, reindeer, elk, dama dama, and white-lipped deer. The invention also relates to an oligonucleotide primer probe composition used for the method. The invention also relates to a GeXP multiple PCR kit containing the composition. The composition is used for performing GeXP multiple PCR detection, and a plurality of deer species can be detected through one-time detection in a simple, rapid, specific, and sensitive mode.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a GeXP multiplex PCR method for qualitative detection of various deer species components, an oligonucleotide primer probe composition for said method, and a composition comprising said combination reagent kits. Background technique [0002] Deer animals and their products have been considered as the best food for health care since ancient times. In 2000, sika deer and red deer were listed as authentic medicinal materials in the Pharmacopoeia of the People's Republic of China. Since then, the application of deer animals and their products in food, drug and health products has gradually increased, such as antler, antler, and antler glue. Venison is also a high-end product among meat products with high nutritional value. Therefore, adulteration of deer ingredients has become an important food inspection item. At present, the methods for species identification a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/16C12Q2537/143Y02A50/30
Inventor 陈颖吴亚君凌胜男杨艳歌刘鸣畅韩建勋
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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