Analysis method for distinguishing citrus chachiensis hortorum from pericarpium citri reticulatae through finger print in combination with stoichiometry

Abstract

The invention discloses an analysis method for distinguishing citrus chachiensis hortorum from pericarpium citri reticulatae through HPLC finger print in combination with stoichiometry. The method comprises the following steps: performing pretreatment on citrus chachiensis hortorum samples and pericarpium citri reticulatae samples respectively, and performing gradient elution through a high performance liquid chromatograph, so as to obtain citrus chachiensis hortorum common mode finger print, analyzing finger print information, so as to obtain 17 common peaks of aurantiamarin taken as a reference peak, and identifying 3 common peaks through comparison of the sample and standard substance; preprocessing HP original data of different batches of citrus chachiensis hortorum samples and pericarpium citri reticulatae samples, and then performing similarity calculation, clustering analysis and principal component analysis. The method has the advantages that analysis results are displayed in modes of similarity data range, tree clustering analysis graphics and PCA score plots, a constructed citrus chachiensis hortorum quality control mode clearly reveals the regularity and difference of citrus chachiensis hortorum from pericarpium citri reticulatae, can visually distinguish varieties of citrus chachiensis hortorum from pericarpium citri reticulatae, relevant verification and analysis are not required, quantitative difference between the varieties is embodied, and the method can serve as an analysis method for distinguishing citrus chachiensis hortorum from pericarpium citri reticulatae.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to an analysis method for distinguishing tangerine peel and tangerine peel by combining fingerprints and chemometrics. Background technique [0002] Guangchenpi is made from the peel of the ripe fruit of Chazhimandarin or dried at low temperature, and it is the authentic medicinal material of Chenpi. Citrus tangerine peel has very high medicinal value, and it is said that "the old one is good". From this point of view, it is of certain significance to effectively identify and distinguish the varieties of tangerine peel for quality control. The Pharmacopoeia distinguishes the wide tangerine peel from the common tangerine peel through character identification, and uses the content of hesperidin as an index for quality control. Although conventional microscopic identification of traits can achieve certain results, this method is relatively primitive, which is not conducive to large-scal...

AI Technical Summary

Problems solved by technology

Although conventional microscopic identification of traits can achieve certain results, this method is relatively primitive, not conducive to large-scale detection, and the detection error is relatively large

[0003] On the other hand, the current quality evaluation of tangerine peel mainly focuses on the detection of one or a few compounds as indicators to determine the quality of medicinal materials. The conclusions are too limited to comprehensively evaluate the quality of medicinal materials.

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