Sample preservation solution, preparation method and applications thereof,

A technology for preserving solutions and samples, which is applied in the field of clinical medicine, can solve the problem of single use of products, achieve good compatibility, simple preparation, and improve the effect of separation

Inactive Publication Date: 2018-06-22
南京福怡科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specimens for pathological diagnosis come from human tissues and exfoliated cells. The specimens need a medium to preserve and fix them during transportation to ensure their integrity. Currently, there are many exfoliated cell preservation solutions, liquid-based cell preservation solutions and tissue fixatives on the market. , the products are basically acidic and have a single use

Method used

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  • Sample preservation solution, preparation method and applications thereof,
  • Sample preservation solution, preparation method and applications thereof,

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A sample preservation solution of the present invention consists of:

[0027] The volume of methanol and ethylene glycol mixed solution is 42%;

[0028] Formaldehyde solution: 10%

[0029] The alkaline substance is 0.01% sodium hydroxide solution;

[0030] 0.1% humectant is butanediol;

[0031] 0.5% potassium chloride solution 10%;

[0032] Water is purified water for in vitro diagnostic reagents.

[0033] A method for preparing a sample preservation solution according to the present invention is prepared by taking 100ml as an example:

[0034] 1) The preparation environment is a 100,000-level purification workshop;

[0035] 2) Methanol and ethylene glycol according to methanol: ethylene glycol = 9: 1, prepare 40ml of mixed solution of methanol and ethylene glycol;

[0036] 3) Add 10ml formaldehyde solution, 10ml 0.5% potassium chloride solution and 0.1ml moisturizer to 2);

[0037] 4) Use 0.01% sodium hydroxide solution and 0.1% acetic acid solution to adjust th...

Embodiment 2

[0044] A sample preservation solution of the present invention consists of:

[0045] The volume of methanol and ethylene glycol mixed solution is 42%;

[0046] Formaldehyde solution: 10%;

[0047] The alkaline substance is 0.01% sodium hydroxide solution;

[0048] 0.1% humectant is butanediol;

[0049] 0.5% potassium chloride solution 10%;

[0050] Water is purified water for in vitro diagnostic reagents.

[0051] A method for preparing a sample preservation solution according to the present invention is prepared by taking 100ml as an example:

[0052] 1) The preparation environment is a 100,000-level purification workshop;

[0053] 2) Methanol and ethylene glycol according to methanol: ethylene glycol = 9: 1, prepare 40ml of mixed solution of methanol and ethylene glycol;

[0054] 3) Add 10ml formaldehyde solution, 10ml 0.5% potassium chloride solution and 0.1ml moisturizer to 2);

[0055] 4) Use 0.01% sodium hydroxide solution and 0.1% acetic acid solution to adjust t...

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Abstract

The invention belongs to the technical field of clinical medicine, and particularly to a sample preservation solution and a preparation method thereof, and a cell smear production method, wherein thesample preservation solution can be used for preservation and fixation of human tissue samples and cast-off cell samples in pathological diagnosis. According to the present invention, the specimen preserved with the sample preservation solution has characteristics of complete cell morphology, complete quantity, uniform distribution, clear chromatography, good adhesion and easy observation and diagnosis.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a sample preservation solution and its preparation method and application. Background technique [0002] Pathological diagnosis is the most reliable of various examination methods for tumors, and it is the final diagnosis of the disease. Specimens for pathological diagnosis come from human tissues and exfoliated cells. The specimens need a medium to preserve and fix them during transportation to ensure their integrity. Currently, there are many exfoliated cell preservation solutions, liquid-based cell preservation solutions and tissue fixatives on the market. , the products are basically acidic and have a single use. Contents of the invention [0003] The object of the present invention is to provide a sample preservation solution and its preparation method and application, which are used for the preservation of histology and cytology samples in pathological diagnosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 姚斌左露露
Owner 南京福怡科技发展股份有限公司
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